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Recombinant Plasmodium falciparum Calcium-dependent protein kinase 1 (CPK1), partial

  • 中文名稱:
    Recombinant Plasmodium falciparum Calcium-dependent protein kinase 1(CPK1) ,partial
  • 貨號:
    CSB-YP351735EWP
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    Recombinant Plasmodium falciparum Calcium-dependent protein kinase 1(CPK1) ,partial
  • 貨號:
    CSB-EP351735EWP
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    Recombinant Plasmodium falciparum Calcium-dependent protein kinase 1(CPK1) ,partial
  • 貨號:
    CSB-EP351735EWP-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    Recombinant Plasmodium falciparum Calcium-dependent protein kinase 1(CPK1) ,partial
  • 貨號:
    CSB-BP351735EWP
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    Recombinant Plasmodium falciparum Calcium-dependent protein kinase 1(CPK1) ,partial
  • 貨號:
    CSB-MP351735EWP
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    CPK1
  • Uniprot No.:
  • 別名:
    CPK1; CDPK1; PFB0815wCalcium-dependent protein kinase 1; EC 2.7.11.1
  • 種屬:
    Plasmodium falciparum (isolate 3D7)
  • 蛋白長度:
    Partial
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Calcium-dependent protein kinase which acts as a sensor and effector of intracellular Ca(2+) levels probably in part downstream of cGMP-activated PKG kinase. By phosphorylating various proteins, required for microneme secretion and thus merozoite egress from and invasion of host erythrocytes. During gametogenesis, essential for the development of both male and female gametes. Phosphorylates SERA5 p50 which enhances SERA5 p50 protease activity; however, SERA5 p50 protease activity has been shown in other studies to be controversial. Probably by phosphorylating SERA5 p50, plays a role in merozoite egress from host erythrocytes. Probably prior or during merozoite invasion of host erythrocytes, phosphorylates rhoptry protein RhopH3 which is required for RhopH3 localization to rhoptries and for its secretion. Probably in late schizonts, phosphorylates myosin A tail domain-interacting protein MTIP and glideosome-associated protein 45 GAP45, both of which are components of the motor complex that generates the force required by the parasite to invade host cells. In late schizonts, phosphorylates inner membrane complex protein IMC1g. In late schizonts, phosphorylates PKA regulatory subunit PKAr in a calcium-dependent manner, which may contribute to the dissociation of regulatory PKAr and catalytic PKAc subunits and promote the activation of PKAc. May phosphorylate raf kinase inhibitory protein RKIP which in turn may regulate CDPK1 catalytic activity. May phosphorylate proteins of the host erythrocyte membranes.
  • 基因功能參考文獻:
    1. critical role in mosquito infection PMID: 29311293
    2. Expression of the J-domain of Plasmodium falciparum CDPK1 results in the arrest of parasite development late in the cell cycle during early schizogony. PMID: 23548171
    3. PfCDPK1 regulates microneme discharge, a key process in erythrocyte invasion by malaria parasites. PMID: 23204525
  • 亞細胞定位:
    Membrane; Lipid-anchor. Cell membrane; Lipid-anchor; Cytoplasmic side. Parasitophorous vacuole membrane; Lipid-anchor. Cytoplasm. Cell projection, cilium, flagellum. Host cell membrane; Lipid-anchor.
  • 蛋白家族:
    Protein kinase superfamily, Ser/Thr protein kinase family, CDPK subfamily
  • 數據庫鏈接:

    KEGG: pfa:PFB0815w



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