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  4. Recombinant Pig Wee1-like protein kinase 2 (WEE2), partial

Recombinant Pig Wee1-like protein kinase 2 (WEE2), partial

  • 中文名稱：
    野豬WEE2重組蛋白
  • 貨號：
    CSB-YP026085PI
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    野豬WEE2重組蛋白
  • 貨號：
    CSB-EP026085PI
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    野豬WEE2重組蛋白
  • 貨號：
    CSB-EP026085PI-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    野豬WEE2重組蛋白
  • 貨號：
    CSB-BP026085PI
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    野豬WEE2重組蛋白
  • 貨號：
    CSB-MP026085PI
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    WEE2; WEE1B; Wee1-like protein kinase 2; EC 2.7.10.2; Wee1-like protein kinase 1B; Wee1B kinase; pWee1B; pigWee1B
  • 種屬：
    Sus scrofa (Pig)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation.
  • 基因功能參考文獻：
    1. results suggest pWEE1B is activated after phosphorylation of the Ser77 residue, which is different from phosphorylation site that activates mWEE1B; that pWEE1B is localized in the nucleus; and that the nuclear localization is essential for its function PMID: 21123961
    2. insufficient amount of Cdc2 and continuous activation of Wee1 B are the cause of meiotic failure of small oocytes in pigs PMID: 19550110
    3. These results suggest that the inhibitory phosphorylation of CDC2, which is catalyzed by pigWee1B, but not pigMyt1, is involved in the meiotic arrest of porcine oocytes. PMID: 19633431
  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family, WEE1 subfamily
  • 組織特異性：
    Ovary-specific.
  • 數(shù)據(jù)庫鏈接：


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