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Recombinant Pig Complement C5a anaphylatoxin (C5)

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  • 中文名稱:
    Recombinant Pig Complement C5a anaphylatoxin(C5)
  • 貨號(hào):
    CSB-YP003995PI
  • 規(guī)格:
    ¥2616
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP003995PI could indicate that this peptide derived from Yeast-expressed Sus scrofa (Pig) C5.
    • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP003995PI could indicate that this peptide derived from Yeast-expressed Sus scrofa (Pig) C5.
  • 其他:

產(chǎn)品詳情

  • 純度:
    Greater than 90% as determined by SDS-PAGE.
  • 基因名:
  • Uniprot No.:
  • 別名:
    C5; Complement C5a anaphylatoxin
  • 種屬:
    Sus scrofa (Pig)
  • 蛋白長度:
    Full Length
  • 來源:
    Yeast
  • 分子量:
    10.6kDa
  • 表達(dá)區(qū)域:
    1-74aa
  • 氨基酸序列
    MLQKKIEEEAAKYKYAMLKKCCYDGAYRNDDETCEERAARIKIGPKCVKAFKDCCYIANQVRAEQSHKNIQLGR
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽:
    N-terminal 6xHis-tagged
  • 產(chǎn)品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲(chǔ)存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項(xiàng):
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述:

    CUSABIO transfected the expression vector which inserted the recombinant DNA into the yeast, cultured the cells, and then induced the transcription and translation of the cloned vector. The N-terminal 6xHis tag sequence was appended to the gene coding for the yeast of the Pig C5 protein to form the recombinant DNA. The recombinant Pig C5 was expressed as N-terminal 6xHis-tagged fusion. The purity of the protein is greater than 90% assayed by SDS-PAGE. It has an apparent molecular weight of approximately 11 kDa.

    C5a, a peptide derived from the fifth component of complement, caused significant prolonged smooth muscle contraction in isolated guinea pig trachea. Earlier studies highlighted an amplifying effect of locally applied C5a on the process; however, the role of systemic complement (C) activation with C5a liberation in blood has not been explored to date. C5a was shown to intensify the allergen-induced anaphylactic crisis in isolated, perfused guinea pig hearts. Inhibitors of C activation or C5a or adenosine action might be useful in preventing or ameliorating C activation-related cardiac anaphylaxis. Many findings unrevealed the effect of C5a anaphylatoxins on guinea-pigisolated blood vessels.

  • Datasheet & COA:
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    Derived from proteolytic degradation of complement C5, C5a anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
  • 亞細(xì)胞定位:
    Secreted.
  • 數(shù)據(jù)庫鏈接:


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