Recombinant Pig 5'-AMP-activated protein kinase subunit gamma-3 (PRKAG3), partial

中文名稱：

野豬PRKAG3重組蛋白
貨號(hào)：

CSB-YP873545PI
規(guī)格：
來(lái)源：

Yeast
其他：

中文名稱：

野豬PRKAG3重組蛋白
貨號(hào)：

CSB-EP873545PI
規(guī)格：
來(lái)源：

E.coli
其他：

中文名稱：

野豬PRKAG3重組蛋白
貨號(hào)：

CSB-EP873545PI-B
規(guī)格：
來(lái)源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

野豬PRKAG3重組蛋白
貨號(hào)：

CSB-BP873545PI
規(guī)格：
來(lái)源：

Baculovirus
其他：

中文名稱：

野豬PRKAG3重組蛋白
貨號(hào)：

CSB-MP873545PI
規(guī)格：
來(lái)源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

PRKAG3
Uniprot No.：

Q9MYP4
別名：

PRKAG35'-AMP-activated protein kinase subunit gamma-3; AMPK gamma3; AMPK subunit gamma-3
種屬：

Sus scrofa (Pig)
蛋白長(zhǎng)度：

Partial
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. AMPK also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. The AMPK gamma3 subunit is a non-catalytic subunit with a regulatory role in muscle energy metabolism. It mediates binding to AMP, ADP and ATP, leading to AMPK activation or inhibition: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.
基因功能參考文獻(xiàn)：
1. Activity of glycogen synthase without the allosteric activator glucose 6-phosphate was decreased in gain of function AMPKgamma3(R200Q) relative to all other genotypes PMID: 27302990
2. Greater net lactate accumulation postmortem (i.e., glycolytic flux) coupled with a lower buffering capacity explains the lower ultimate pH of meat from AMPKgamma3(R200Q) mutant pigs. PMID: 26241465
3. allelic frequencies of PRKAG3 in several pig breeds PMID: 24074175
4. The variation in the promoter region of the porcine PRKAG3 gene has associations with meat quality phenotypes, including traits which are influenced by glycolytic potential and muscle metabolism in a breed-dependent manner. PMID: 22831392
5. PRKAG3 mutation influences muscle characteristics and promotes an oxidative phenotype to a varying degree among muscles with different functions PMID: 21435205
6. increased rate of glycogen synthesis following exercise in pigs carrying the PRKAG3 mutation correlates with an increased signalling response of Akt and its substrate, AS160. PMID: 20028849
7. Genetic variation of PRKAG3 was studied in indigenous breeds of China. PMID: 15231235
8. the R225Q mutation of PRKAG3 is associated with higher basal AMPK activity and diminished AMP dependence PMID: 15247217
9. findings suggest that the R200Q mutation results in a constitutively active enzyme, leading to hyperaccumulation of glycogen due to the increased influx of glucose PMID: 15274139
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相關(guān)疾病：

Defects in PRKAG3 are the cause of the RN- phenotype which is associated with excess glycogen content (about 70%) in skeletal muscle. This mutation originated in the hampshire breed pigs and has beneficial effects on meat content but detrimental effects on processing yield.
蛋白家族：

5'-AMP-activated protein kinase gamma subunit family
組織特異性：

Muscle.
數(shù)據(jù)庫(kù)鏈接：

KEGG: ssc:397149

STRING: 9823.ENSSSCP00000017163

UniGene: Ssc.287