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  4. Recombinant Pig 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase (HSD3B), partial

Recombinant Pig 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase (HSD3B), partial

  • 中文名稱：
    野豬HSD3B重組蛋白
  • 貨號：
    CSB-YP863078PI1
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    野豬HSD3B重組蛋白
  • 貨號：
    CSB-EP863078PI1
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    野豬HSD3B重組蛋白
  • 貨號：
    CSB-EP863078PI1-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    野豬HSD3B重組蛋白
  • 貨號：
    CSB-BP863078PI1
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    野豬HSD3B重組蛋白
  • 貨號：
    CSB-MP863078PI1
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    HSD3B
  • Uniprot No.：
  • 別名：
    HSD3B; 3b-HSD; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase; 3-beta-HSD [Includes: 3-beta-hydroxy-Delta(5-steroid dehydrogenase; 3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase; Steroid Delta-isomerase; Delta-5-3-ketosteroid isomerase]
  • 種屬：
    Sus scrofa (Pig)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.
  • 基因功能參考文獻：
    1. It was concluded that the presence of apelin/APLNR in the CL of pigs and stimulatory effects of apelin on progesterone secretion and 3beta-HSD levels suggest potential auto/paracrine regulation by apelin in the luteal phase of the estrous cycle. PMID: 29576467
    2. Glucocorticoid receptor binding to 3beta-HSD promoter is involved in the differential hepatic 3beta-HSD expression between boars and barrows. PMID: 28877400
    3. We found that adiponectin and insulin alone regulates the expression of StAR, CYP11A1 and HSD3B1 genes and secretion of P4 and A4 by the porcine endometrial and myometrial tissue explants during early pregnancy and the oestrous cycle. PMID: 27512005
    4. The effects of androgen blockade on the expression of 3beta-hydroxysteroid dehydrogenase (3beta-HSD), CYP17, and 17beta-hydroxysteroid dehydrogenase type 1 (17beta-HSD1) in the porcine fetus are reported. PMID: 25130044
    5. Study found that uncastrated Duroc pigs with the GG SNP5 genotype had significantly higher 3beta-HSD mRNA levels than did uncastrated Duroc pigs with other SNP5 genotypes. PMID: 23581486
    6. New insights to the transcriptional regulation of 3betaHSD1 and 17betaHSD7 and further hints to their involvement in steroid metabolism. PMID: 22405929
    7. Significant sequence variations were found in the 5'flanking region of the 3beta-HSD gene, where differences in the number of TTAT repeats and 3 SNP were observed in the different breeds PMID: 17060418
    8. The expression of 3betaHSD mRNA was detected by the use of RT-PCR technique in all investigated samples isolated at different stages of pregnancy. PMID: 17647136
    9. Genistein and daidzein have direct effects on porcine granulosa cell progesterone synthesis involving the inhibition of 3-beta-HSD enzyme activity. PMID: 17728101

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  • 亞細胞定位：
    Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein.
  • 蛋白家族：
    3-beta-HSD family
  • 數據庫鏈接：

    KEGG: ssc:445539

    UniGene: Ssc.14393



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