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  4. Recombinant Peptide-N (4)- (N-acetyl-beta-glucosaminyl)asparagine amidase (png-1), partial

Recombinant Peptide-N (4)- (N-acetyl-beta-glucosaminyl)asparagine amidase (png-1), partial

  • 中文名稱：
    秀麗隱桿線蟲png-1重組蛋白
  • 貨號：
    CSB-YP883315CXY
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲png-1重組蛋白
  • 貨號：
    CSB-EP883315CXY
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲png-1重組蛋白
  • 貨號：
    CSB-EP883315CXY-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲png-1重組蛋白
  • 貨號：
    CSB-BP883315CXY
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲png-1重組蛋白
  • 貨號：
    CSB-MP883315CXY
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    png-1
  • Uniprot No.：
  • 別名：
    png-1; F56G4.5; Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase; EC 3.5.1.52; Peptide:N-glycanase; PNGase
  • 種屬：
    Caenorhabditis elegans
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins. Also displays oxidoreductase (thioredoxin) activity. Involved in regulating the expression of proteasomal subunits such as rpt-3 in order to confer resistance to proteasomal dysfunction.
  • 基因功能參考文獻：
    1. Caenorhabditis elegans PNG-1 exhibits dual enzyme functions, not only as PNGase but also as an oxidoreductase (thioredoxin). PMID: 17509531
    2. Authors show a developmental role for a PNGase and Rad-23 in the regulation of neuronal branching during organ innervation. PMID: 20130186
  • 亞細胞定位：
    Cytoplasm. Endoplasmic reticulum. Note=ER localization inferred from partial colocalization with an ER marker, membrane protein PIG-X.
  • 蛋白家族：
    Transglutaminase-like superfamily, PNGase family
  • 數據庫鏈接：

    KEGG: cel:CELE_F56G4.5

    STRING: 6239.F56G4.5.1

    UniGene: Cel.18605



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