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  4. Recombinant Neurospora crassa Histone-lysine N-methyltransferase, H3 lysine-9 specific dim-5 (dim-5)

Recombinant Neurospora crassa Histone-lysine N-methyltransferase, H3 lysine-9 specific dim-5 (dim-5)

  • 中文名稱：
    小神經孢子蟲dim-5重組蛋白
  • 貨號：
    CSB-YP848857NHA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小神經孢子蟲dim-5重組蛋白
  • 貨號：
    CSB-EP848857NHA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小神經孢子蟲dim-5重組蛋白
  • 貨號：
    CSB-EP848857NHA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小神經孢子蟲dim-5重組蛋白
  • 貨號：
    CSB-BP848857NHA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小神經孢子蟲dim-5重組蛋白
  • 貨號：
    CSB-MP848857NHA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    dim-5
  • Uniprot No.：
  • 別名：
    dim-5; 29E8.110; NCU04402Histone-lysine N-methyltransferase; H3 lysine-9 specific dim-5; EC 2.1.1.355; Histone H3-K9 methyltransferase dim-5; H3-K9-HMTase dim-5; HKMT
  • 種屬：
    Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-331
  • 氨基酸序列
    MEKAFRPHFF NHGKPDANPK EKKNCHWCQI RSFATHAQLP ISIVNREDDA FLNPNFRFID HSIIGKNVPV ADQSFRVGCS CASDEECMYS TCQCLDEMAP DSDEEADPYT RKKRFAYYSQ GAKKGLLRDR VLQSQEPIYE CHQGCACSKD CPNRVVERGR TVPLQIFRTK DRGWGVKCPV NIKRGQFVDR YLGEIITSEE ADRRRAESTI ARRKDVYLFA LDKFSDPDSL DPLLAGQPLE VDGEYMSGPT RFINHSCDPN MAIFARVGDH ADKHIHDLAL FAIKDIPKGT ELTFDYVNGL TGLESDAHDP SKISEMTKCL CGTAKCRGYL W
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Histone methyltransferase that specifically trimethylates histone H3 to form H3K9me3. H3K9me3 marks chromatin regions for DNA methylation. Dim-5 recognizes Arg-8 to Gly-12 of the H3 tail with Thr-11 and Gly-12 being the most important specificity determinants, the recognition of whcih is important to distinguish H3K9 from H3K27 and H4K20.
  • 基因功能參考文獻：
    1. DIM-5 modifies chromatin at frequency and represses light-activated gene expression PMID: 25429045
    2. Phe at the position equivalent to Phe(281) of Neurospora crassa DIM-5 or Phe(1205) of human G9a allows the enzyme to perform di and tri-methylation, whereas a Tyr at this position is restrictive, inhibiting tri-methylation and yielding a mono- or di-MTase PMID: 15590646
  • 亞細胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, Suvar3-9 subfamily
  • 數據庫鏈接：

    KEGG: ncr:NCU04402



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