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  4. Recombinant Neisseria meningitidis serogroup B CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase (lst)

Recombinant Neisseria meningitidis serogroup B CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase (lst)

  • 中文名稱：
    腦膜炎奈瑟菌血清組lst重組蛋白
  • 貨號：
    CSB-YP304115NGG
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    腦膜炎奈瑟菌血清組lst重組蛋白
  • 貨號：
    CSB-EP304115NGG
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    腦膜炎奈瑟菌血清組lst重組蛋白
  • 貨號：
    CSB-EP304115NGG-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    腦膜炎奈瑟菌血清組lst重組蛋白
  • 貨號：
    CSB-BP304115NGG
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    腦膜炎奈瑟菌血清組lst重組蛋白
  • 貨號：
    CSB-MP304115NGG
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    lst
  • Uniprot No.：
  • 別名：
    lst; NMB0922; N-acetyllactosaminide alpha-2,3-sialyltransferase; EC 2.4.99.6; CMP-N-acetylneuraminate:beta-galactoside alpha-2,3-sialyltransferase; CMP-Neu5Ac:beta-galactoside alpha-2,3-sialyltransferase; Lipooligosaccharide sialyltransferase; LOS sialyltransferase
  • 種屬：
    Neisseria meningitidis serogroup B (strain MC58)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-371
  • 氨基酸序列
    MGLKKACLTV LCLIVFCFGI FYTFDRVNQG ERNAVSLLKE KLFNEEGEPV NLIFCYTILQ MKVAERIMAQ HPGERFYVVL MSENRNEKYD YYFNQIKDKA ERAYFFHLPY GLNKSFNFIP TMAELKVKSM LLPKVKRIYL ASLEKVSIAA FLSTYPDAEI KTFDDGTGNL IQSSSYLGDE FSVNGTIKRN FARMMIGDWS IAKTRNASDE HYTIFKGLKN IMDDGRRKMT YLPLFDASEL KTGDETGGTV RILLGSPDKE MKEISEKAAK NFKIQYVAPH PRQTYGLSGV TTLNSPYVIE DYILREIKKN PHTRYEIYTF FSGAALTMKD FPNVHVYALK PASLPEDYWL KPVYALFTQS GIPILTFDDK N
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Catalyzes the transfer of sialic acid from the substrate CMP-N-acetylneuraminate to the terminal galactose residue of the lacto-N-neotetraose branch of surface lipooligosaccharide (LOS), forming an alpha-2,3-sialyl linkage. Thus, functions in the sialylation of LOS, which plays a role in the evasion of the host immune response by protecting N.meningitidis from complement-mediated serum killing and from phagocytic killing by neutrophils. In vitro, can use a number of different synthetic acceptors with lactose or galactose as the saccharide portion, but shows a strong preference for the N-acetyllactosamine containing acceptor.
  • 基因功能參考文獻：
    1. Coexpression with the neuC, neuB and neuA Campylobacter jejuni (ATCC 43438) genes in E. coli K12 devoid of Neu5Ac aldolase and of ManNAc kinase was shown to efficiently produce 3'sialyllactose PMID: 18378033
  • 亞細胞定位：
    Cell outer membrane; Peripheral membrane protein; Extracellular side.
  • 蛋白家族：
    Glycosyltransferase 52 family
  • 數據庫鏈接：

    KEGG: nme:NMB0922

    STRING: 122586.NMB0922



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