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Recombinant Naja kaouthia Cobra venom factor, partial

In Stock
  • 中文名稱:
    Recombinant Naja kaouthia Cobra venom factor,partial
  • 貨號:
    CSB-EP856221NAF
  • 規格:
    ¥2328
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

產品詳情

  • 純度:
    Greater than 90% as determined by SDS-PAGE.
  • 基因名:
    N/A
  • Uniprot No.:
  • 別名:
    ; Cobra venom factor; CVF; CVFk; Complement C3 homolog) [Cleaved into: Cobra venom factor alpha chain; Cobra venom factor gamma chain; Cobra venom factor beta chain]
  • 種屬:
    Naja kaouthia (Monocled cobra) (Naja siamensis)
  • 蛋白長度:
    Partial
  • 來源:
    E.coli
  • 分子量:
    44.4kDa
  • 表達區域:
    733-984aa
  • 氨基酸序列
    DDNEDGFIADSDIISRSDFPKSWLWLTKDLTEEPNSQGISSKTMSFYLRDSITTWVVLAVSFTPTKGICVAEPYEIRVMKVFFIDLQMPYSVVKNEQVEIRAILHNYVNEDIYVRVELLYNPAFCSASTKGQRYRQQFPIKALSSRAVPFVIVPLEQGLHDVEIKASVQEALWSDGVRKKLKVVPEGVQKSIVTIVKLDPRAKGVGGTQLEVIKARKLDDRVPDTEIETKIIIQGDPVAQIIENSIDGSKLN
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    N-terminal 6xHis-SUMO-tagged
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產品描述:

    The synthesis of Recombinant N.kaouthia CVF protein began with the isolation of CVF gene from the native genome. The next step was to amplify the CVF gene by inserting the CVF gene into an expression vector and then introducing the vector into the selected host cell, E.coli. Significant amounts of recombinant N.kaouthia CVF protein were produced by the host only when expression genes were added. For the purpose of protein purification, the vector contains N-terminal 6xHis-SUMO tag to the CVF DNA sequence. The purity of this protein is 90%+ determined by SDS-PAGE.

    Cobra venom factor (CVF or CVFk), also known as Complement C3 homolog is a complement-activating protein in cobra venom. It can be cleaved into the three chains: cobra venom factor alpha chain, cobra venom factor gamma chain and cobra venom factor beta chain. A functional analog of CVF in human is complement component C3b (a active fragment of C3). Similar to C3b, CVF binds factor B, which is then cleaved by factor D, giving rise to the CVFBb complex that targets the same scissile bond in C3 as the authentic complement convertases C4bC2a and C3bBb.

  • Datasheet & COA:
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Complement-activating protein in cobra venom. It is a structural and functional analog of complement component C3b, the activated form of C3. It binds factor B (CFB), which is subsequently cleaved by factor D (CFD) to form the bimolecular complex CVF/Bb. CVF/Bb is a C3/C5 convertase that cleaves both complement components C3 and C5. Structurally, it resembles the C3b degradation product C3c, which is not able to form a C3/C5 convertase. Unlike C3b/Bb, CVF/Bb is a stable complex and completely resistant to the actions of complement regulatory factors H (CFH) and I (CFI). Therefore, CVF continuously activates complement resulting in the depletion of complement activity.
  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    Venom complement C3 homolog family
  • 組織特異性:
    Expressed by the venom gland.


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