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  4. Recombinant NEDD8-activating enzyme E1 catalytic subunit (uba-3)

Recombinant NEDD8-activating enzyme E1 catalytic subunit (uba-3)

  • 中文名稱：
    秀麗隱桿線蟲rfl-1重組蛋白
  • 貨號：
    CSB-YP613898CXY
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲rfl-1重組蛋白
  • 貨號：
    CSB-EP613898CXY
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲rfl-1重組蛋白
  • 貨號：
    CSB-EP613898CXY-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲rfl-1重組蛋白
  • 貨號：
    CSB-BP613898CXY
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲rfl-1重組蛋白
  • 貨號：
    CSB-MP613898CXY
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    rfl-1
  • Uniprot No.：
  • 別名：
    rfl-1; uba-3; F11H8.1; NEDD8-activating enzyme E1 catalytic subunit; EC 6.2.1.-; Ectopic membrane ruffles in embryo protein 1; Ubiquitin-activating enzyme 3 homolog
  • 種屬：
    Caenorhabditis elegans
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-430
  • 氨基酸序列
    MVSVDPLATE RWRSIRRLTD RDSAYKVPWF VPGPENFEAL QNTKILVIGA GGLGCELLKN LALSGFRTIE VIDMDTIDVS NLNRQFLFRE SDVGKSKAEV AAAFVQQRVV GCQVTAHNCR IEDKGQEFYR KFSIIICGLD SIPARRWING MLCDLVLEMA DGKPDENTII PMIDGGTEGF KGNARVIYPK FTACIDCTLD LYPPQVNFPL CTIAHTPRLP EHCIEYIKVV VWPEEKPFEG VSLDADDPIH VEWVLERASL RAEKYNIRGV DRRLTSGVLK RIIPAVASTN AVIAASCALE ALKLATNIAK PIDNYLNFTQ IHGAYTSVVS MMKDDNCLTC SGGRLPFEVS PSSTLESLII RLSERFHLKH PTLATSTRKL YCISSFMPQF EQESKENLHT SMKDLVSDGE EILVSDEALS RALTLRIQLI
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Catalytic subunit of the dimeric rfl-1 (uba-3)-ula-1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-uba-3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of ubc-12. Required for cytokinesis and mitotic spindle orientation during early embryogenesis.
  • 基因功能參考文獻：
    1. Depletion of the strongest specific suppressors rescued the early embryonic cell division defects in rfl-1 mutants; depletion of the two strongest specific enhancers did not affect the early embryonic cell division defects PMID: 19528325
  • 亞細胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    Ubiquitin-activating E1 family, UBA3 subfamily
  • 組織特異性：
    Expressed in intestine, vulva epithelium and head and tail neurons.
  • 數據庫鏈接：

    KEGG: cel:CELE_F11H8.1

    STRING: 6239.F11H8.1.1

    UniGene: Cel.10144



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