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Recombinant Mycobacterium smegmatis Enoyl-[acyl-carrier-protein] reductase [NADH] (inhA)

  • 中文名稱:
    Recombinant Mycobacterium smegmatis Enoyl-[acyl-carrier-protein] reductase [NADH](inhA)
  • 貨號:
    CSB-YP337231MVX
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    Recombinant Mycobacterium smegmatis Enoyl-[acyl-carrier-protein] reductase [NADH](inhA)
  • 貨號:
    CSB-EP337231MVX
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    Recombinant Mycobacterium smegmatis Enoyl-[acyl-carrier-protein] reductase [NADH](inhA)
  • 貨號:
    CSB-EP337231MVX-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    Recombinant Mycobacterium smegmatis Enoyl-[acyl-carrier-protein] reductase [NADH](inhA)
  • 貨號:
    CSB-BP337231MVX
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    Recombinant Mycobacterium smegmatis Enoyl-[acyl-carrier-protein] reductase [NADH](inhA)
  • 貨號:
    CSB-MP337231MVX
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    inhA
  • Uniprot No.:
  • 別名:
    inhA; MSMEG_3151; MSMEI_3070; Enoyl-[acyl-carrier-protein] reductase [NADH]; ENR; Enoyl-ACP reductase; EC 1.3.1.9; FAS-II enoyl-ACP reductase; NADH-dependent 2-trans-enoyl-ACP reductase
  • 種屬:
    Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
  • 蛋白長度:
    full length protein
  • 表達區域:
    1-269
  • 氨基酸序列
    MTGLLEGKRI LVTGIITDSS IAFHIAKVAQ EAGAELVLTG FDRLKLVKRI ADRLPKPAPL LELDVQNEEH LSTLADRITA EIGEGNKIDG VVHSIGFMPQ SGMGINPFFD APYEDVSKGI HISAYSYASL AKAVLPIMNP GGGIVGMDFD PTRAMPAYNW MTVAKSALES VNRFVAREAG KVGVRSNLVA AGPIRTLAMS AIVGGALGDE AGQQMQLLEE GWDQRAPLGW NMKDPTPVAK TVCALLSDWL PATTGTVIYA DGGASTQLL
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Enoyl-ACP reductase of the type II fatty acid syntase (FAS-II) system, which is involved in the biosynthesis of mycolic acids, a major component of mycobacterial cell walls. Catalyzes the NADH-dependent reduction of the double bond of 2-trans-enoyl-[acyl-carrier protein], an essential step in the fatty acid elongation cycle of the FAS-II pathway. Shows preference for long-chain fatty acyl thioester substrates (>C16), and can also use 2-trans-enoyl-CoAs as alternative substrates. The mycobacterial FAS-II system utilizes the products of the FAS-I system as primers to extend fatty acyl chain lengths up to C56, forming the meromycolate chain that serves as the precursor for final mycolic acids.; Is the primary target of the first-line antitubercular drug isoniazid (INH) and of the second-line drug ethionamide (ETH). Overexpressed inhA confers INH and ETH resistance to M.smegmatis. The mechanism of isoniazid action against InhA is covalent attachment of the activated form of the drug to the nicotinamide ring of NAD and binding of the INH-NAD adduct to the active site of InhA. Similarly, the ETH-NAD adduct binds InhA.
  • 亞細胞定位:
    Secreted, cell wall.
  • 蛋白家族:
    Short-chain dehydrogenases/reductases (SDR) family, FabI subfamily
  • 數據庫鏈接:


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