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  4. Recombinant Murine coronavirus Replicase polyprotein 1ab (rep)

Recombinant Murine coronavirus Replicase polyprotein 1ab (rep)

  • 中文名稱：
    Recombinant Murine coronavirus Replicase polyprotein 1ab(rep)
  • 貨號(hào)：
    CSB-YP326648MJZ
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Murine coronavirus Replicase polyprotein 1ab(rep)
  • 貨號(hào)：
    CSB-EP326648MJZ
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Murine coronavirus Replicase polyprotein 1ab(rep)
  • 貨號(hào)：
    CSB-EP326648MJZ-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Murine coronavirus Replicase polyprotein 1ab(rep)
  • 貨號(hào)：
    CSB-BP326648MJZ
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Murine coronavirus Replicase polyprotein 1ab(rep)
  • 貨號(hào)：
    CSB-MP326648MJZ
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    rep
  • Uniprot No.：
  • 別名：
    rep; 1a-1b; Replicase polyprotein 1ab; pp1ab; ORF1ab polyprotein) [Cleaved into: Non-structural protein 1-non-structural protein 3 fusion; nsp1-nsp3 fusion)]; Fragment
  • 種屬：
    Murine coronavirus (strain defective JHM) (MHV) (Murine hepatitis virus)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-500
  • 氨基酸序列
    MAKMGKYGLG FKWAPEFPWM LPNASEKLGN PERSEEDGFC PSAAQEPKVK GKTLVNHVRV DCSRLPALEC CVQSAIIRDI FVDEDPQKVE ASTMMALQFG SAVLVKPSKR LSVQAWAKLG VLPKTPAMGL FKRFCLCNTR ECVCDAHVAF QLFTVQPDGV CLGNGRFIGW FVPVTAIPEY AKQWLQPWSI LLRKGGNKGS VTSGHSRRAV TMPVYDFNAT DVVYADENQD DDADDPVVLV ADTQEEDGVA KEQVDSADSE ICVAHTVGQE MTEPDAVGSQ TPIASAEETE VGEACDREGI AEVKATVCAD ALDACPDQVE AFDIEKVEDS ILSELQTELN APADKTYEDV LAFDAIYSET LSAFYAVPSD ETHFKVCGFY SPAIERTNCW LRSTLIVMQS LPLEFKDLGM QKLWLSYKAG YDQCFVDKLV KSAPKSIILP QGGYVADFAY FFLSQCSFKV HANWRCLKCG MELKLQGLDA VFFYGDVVSH MCKCSFKAYF
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.; The papain-like proteinase 1 (PL1-PRO) is responsible for the cleavages located at the N-terminus of the replicase polyprotein. Activity of PL1-PRO is strongly dependent on zinc.; Non-structural protein 1: binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.
  • 蛋白家族：
    Coronaviruses polyprotein 1ab family


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