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Recombinant Murine coronavirus Nucleoprotein (N)

  • 中文名稱:
    Recombinant Murine coronavirus Nucleoprotein(N)
  • 貨號:
    CSB-YP361047MJY
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    Recombinant Murine coronavirus Nucleoprotein(N)
  • 貨號:
    CSB-EP361047MJY
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    Recombinant Murine coronavirus Nucleoprotein(N)
  • 貨號:
    CSB-EP361047MJY-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    Recombinant Murine coronavirus Nucleoprotein(N)
  • 貨號:
    CSB-BP361047MJY
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    Recombinant Murine coronavirus Nucleoprotein(N)
  • 貨號:
    CSB-MP361047MJY
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
  • Uniprot No.:
  • 別名:
    N; 7a; Nucleoprotein; Nucleocapsid protein; NC; Protein N
  • 種屬:
    Murine coronavirus (strain A59) (MHV-A59) (Murine hepatitis virus)
  • 蛋白長度:
    full length protein
  • 表達區域:
    1-454
  • 氨基酸序列
    MSFVPGQENA GGRSSSVNRA GNGILKKTTW ADQTERGPNN QNRGRRNQPK QTATTQPNSG SVVPHYSWFS GITQFQKGKE FQFAEGQGVP IANGIPASEQ KGYWYRHNRR SFKTPDGQQK QLLPRWYFYY LGTGPHAGAS YGDSIEGVFW VANSQADTNT RSDIVERDPS SHEAIPTRFA PGTVLPQGFY VEGSGRSAPA SRSGSRSQSR GPNNRARSSS NQRQPASTVK PDMAEEIAAL VLAKLGKDAG QPKQVTKQSA KEVRQKILNK PRQKRTPNKQ CPVQQCFGKR GPNQNFGGSE MLKLGTSDPQ FPILAELAPT VGAFFFGSKL ELVKKNSGGA DEPTKDVYEL QYSGAVRFDS TLPGFETIMK VLNENLNAYQ KDGGADVVSP KPQRKGRRQA QEKKDEVDNV SVAKPKSSVQ RNVSRELTPE DRSLLAQILD DGVVPDGLED DSNV
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.
  • 基因功能參考文獻:
    1. Coronavirus N phosphorylation by GSK-3 allows recruitment of the RNA helicase DDX1, which facilitates template readthrough and enables longer viral positive-sense single-stranded genomic RNA synthesis. PMID: 25299332
    2. The second domain of N protein plays an essential role in recognizing the RNA structure that allows the selective packaging of genomic RNA into assembled virions. PMID: 24501403
    3. These results support a model in which the N-nsp3 interaction serves to tether the genome to the newly translated replicase-transcriptase complex at a very early stage of infection. PMID: 23760243
    4. Structures of the N- and C-terminal domains of MHV-A59 nucleocapsid protein PMID: 21203940
    5. analysis of the MHV-CoV A59 nucleocapsid protein COOH-terminal negative charges PMID: 17037518
    6. analysis of mouse hepatitis coronavirus nucleocapsid phosphorylation PMID: 17037524
    7. Two domains of N protein are critical for M protein recognition during virus assembly. PMID: 19420077

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  • 亞細胞定位:
    Virion. Host endoplasmic reticulum-Golgi intermediate compartment. Host Golgi apparatus.
  • 蛋白家族:
    Betacoronavirus nucleocapsid protein family
  • 數據庫鏈接:

    KEGG: vg:1489757



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