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  4. Recombinant Mouse mRNA-decapping enzyme 2 (Dcp2)

Recombinant Mouse mRNA-decapping enzyme 2 (Dcp2)

  • 中文名稱：
    Recombinant Mouse mRNA-decapping enzyme 2(Dcp2)
  • 貨號(hào)：
    CSB-YP887471MO
  • 說(shuō)明書：
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Mouse mRNA-decapping enzyme 2(Dcp2)
  • 貨號(hào)：
    CSB-EP887471MO
  • 說(shuō)明書：
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Mouse mRNA-decapping enzyme 2(Dcp2)
  • 貨號(hào)：
    CSB-EP887471MO-B
  • 說(shuō)明書：
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Mouse mRNA-decapping enzyme 2(Dcp2)
  • 貨號(hào)：
    CSB-BP887471MO
  • 說(shuō)明書：
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Mouse mRNA-decapping enzyme 2(Dcp2)
  • 貨號(hào)：
    CSB-MP887471MO
  • 說(shuō)明書：
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Dcp2
  • Uniprot No.：
  • 別名：
    Dcp2m7GpppN-mRNA hydrolase; EC 3.6.1.62; mRNA-decapping enzyme 2
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-422
  • 氨基酸序列
    MEPKRLEIPG SVLDDLCSRF ILHIPSEERD NAIRVCFQIE LAHWFYLDFY MQNTPGLPQC GIRDFAKAVF SHCPFLLPQG EDVEKILDEW KEYKMGVPTY GAIILDETLE NVLLVQGYLA KSGWGFPKGK VNKEEAPHDC AAREVFEETG FDIKDYICKD DYIELRINDQ LARLYIIPGV PKDTKFNPKT RREIRNIEWF SIEKLPCHRN DMTPKSKLGL APNKFFMAIP FIRPLRDWLS RRFGDSSDSD NGFSSAGSTP ARPTVEKLSR TKFRHSQQLF PEGSPSDQWV KHRQPLQQKS HSNHGEVSDL LKAKNQNMRG NGRKQYQDSP NQKKRANGVH GQPAKQQNPL VKCEKKLHPR KLQDNFETDA TCDLPCSGEE PSVEHAEGHS VACNGHCKFP FSSRAFLSFK FDQNAIMKIL DL
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Decapping metalloenzyme that catalyzes the cleavage of the cap structure on mRNAs. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Plays a role in replication-dependent histone mRNA degradation. Has higher activity towards mRNAs that lack a poly(A) tail. Has no activity towards a cap structure lacking an RNA moiety. The presence of a N(6)-methyladenosine methylation at the second transcribed position of mRNAs (N(6),2'-O-dimethyladenosine cap; m6A(m)) provides resistance to DCP2-mediated decapping. Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts.
  • 基因功能參考文獻(xiàn)：
    1. results suggest miR-141-3p/200a-3p may be involved in post-transcriptional repression of Dcp2 expression during renal development PMID: 29912646
    2. ERK-phosphorylated Dcp1a enhances its interaction with the decapping enzyme Dcp2 during early differentiation of 3T3-L1 cells. PMID: 23637887
    3. Recruitment of DCP1A and DCP2 increases the mRNA degradation capacity of the maturing oocyte so that by the 2-cell stage, most of the maternal mRNA is degraded. PMID: 23221396
    4. The results showed that DCP1A and DCP2 are critical in the transition from mRNA stability to instability during meiotic maturation and that mRNA degradation must be successful to execute the oocyte-to-zygote transition. PMID: 23136299
    5. In this study the increase in Irf-7 mRNA within the background of reduced Dcp2 levels was attributed to a stabilization of the Irf-7 mRNA, suggesting that Dcp2 normally modulates Irf-7 mRNA stability. PMID: 22252322
    6. Like Dcp2, Nudt16 also regulates the stability of a subset of mRNAs including a member of the motin family of proteins involved in angiogenesis. PMID: 21070968

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  • 亞細(xì)胞定位：
    Cytoplasm, P-body. Nucleus.
  • 蛋白家族：
    Nudix hydrolase family, DCP2 subfamily
  • 組織特異性：
    Strongly expressed in brain and testis. Weakly expressed in lung. Not detected in heart, liver, kidney and muscle (at protein level).
  • 數(shù)據(jù)庫(kù)鏈接：


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