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  4. Recombinant Mouse Vacuolar protein sorting-associated protein 33B (Vps33b)

Recombinant Mouse Vacuolar protein sorting-associated protein 33B (Vps33b)

  • 中文名稱：
    小鼠Vps33b重組蛋白
  • 貨號：
    CSB-YP025903MO
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Vps33b重組蛋白
  • 貨號：
    CSB-EP025903MO
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Vps33b重組蛋白
  • 貨號：
    CSB-EP025903MO-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Vps33b重組蛋白
  • 貨號：
    CSB-BP025903MO
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Vps33b重組蛋白
  • 貨號：
    CSB-MP025903MO
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Vps33b
  • Uniprot No.：
  • 別名：
    Vps33b; Vacuolar protein sorting-associated protein 33B
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    2-617
  • 氨基酸序列
    AFPHRLDAPELPDFSMLKRLARDQLIYLLEQLPGKKDLFIEADLMSPLDRIANVSILKQH EVDKLYKVENKPALSANEQLCFLVRPRIKNMRYIASLVNADKLAGRIRKYKVILSPQKFY ACEMVLEEEGVYGDVSCDEWAFSLLPLDVDLLSMELPEFFRDYFLEGDQRWINTVAQALH LLSTLYGPFPNCYGIGRCAKMSYDLWRKLEEEEDSETKGRKPEIGHIFLLDRDVDFVTAL CSQVVYEGLVDDTFRIKCGSVDFGPEVTSSDKSLKVLLNAEDKVFSEIRNEHFSNVFGFL SQKARNLQAQYDRRRGMDIKQMKNFVSQELKGLKQEHRLLSLHIGACESIMKKKTKQDFQ ELIKTEHALLEGFNIRESTSYIEEHIDRQVSPIESLRLMCLLSITENGLIPKDYRSLKTQ YLQSYGPEHLLTFSNLRRAGLLTEQAPGDTLTAVESKVSKLVTDKAAGKITDAFSSLAKR SNFRAISKKLNLIPRVDGEYDLKVPRDMAYVFSGAYVPLSCRIIEQVLDRRSWQGLDEVV RLLNCSEFAFTDTAKEDKASSESLRLILVVFLGGCTFSEISALRFLGREKGYRFIFLTTA VTNSARLMEAMSEVKS
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. Mediates phagolysosomal fusion in macrophages. Proposed to be involved in endosomal maturation implicating in part VIPAS39. In epithelial cells, the VPS33B:VIPAS39 complex may play a role in the apical RAB11A-dependentrecycling pathway and in the maintenance of the apical-basolateral polarity. Seems to be involved in the sorting of specific cargos from the trans-Golgi network to alpha-granule-destined multivesicular bodies (MVBs) promoting MVBs maturation in megakaryocytes.
  • 基因功能參考文獻：
    1. VPS33B is vital for maintenance of mammalian structural and functional hepatocyte polarity. PMID: 28082148
    2. this study shows that Vps33B is required in mouse macrophages for normal maturation of phagosomes and specialized endosomes that are induced upon pattern-recognition receptors activation PMID: 27496733
    3. VPS33B interacted with the GDI2/RAB11A/RAB27A pathway to regulate the trafficking of secretory proteins as exosomes. PMID: 27797340
    4. The data presented in this study suggest that Vps33b is involved in alpha-granule formation possibly by facilitating the Vwf-positive vesicular trafficking to alpha-granule-related vacuoles in megakaryocytes. PMID: 27319744
    5. Abnormal protein trafficking and impairment in multivesicular bodies maturation in Megakaryocytes underlie the alpha-granule deficiency in Vps33b(fl/fl)-ER(T2) mouse and ARC patients. PMID: 25947942

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  • 亞細胞定位：
    Late endosome membrane; Peripheral membrane protein; Cytoplasmic side. Lysosome membrane; Peripheral membrane protein; Cytoplasmic side. Early endosome. Cytoplasmic vesicle, clathrin-coated vesicle. Recycling endosome.
  • 蛋白家族：
    STXBP/unc-18/SEC1 family
  • 數據庫鏈接：


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