Recombinant Mouse Three-prime repair exonuclease 1 (Trex1)

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中文名稱(chēng)：

小鼠Trex1重組蛋白
品名簡(jiǎn)稱(chēng)：

Recombinant Mouse Trex1 protein
貨號(hào)：

CSB-EP821009MO
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

Trex1
Uniprot No.：

Q91XB0
別名：

Trex1; Three-prime repair exonuclease 1; EC 3.1.11.2; 3'-5' exonuclease TREX1
種屬：

Mus musculus (Mouse)
蛋白長(zhǎng)度：

Full length
來(lái)源：

E.coli
分子量：

41.1 kDa
表達(dá)區(qū)域：

1-314aa
氨基酸序列

MGSQTLPHGHMQTLIFLDLEATGLPSSRPEVTELCLLAVHRRALENTSISQGHPPPVPRPPRVVDKLSLCIAPGKACSPGASEITGLSKAELEVQGRQRFDDNLAILLRAFLQRQPQPCCLVAHNGDRYDFPLLQTELARLSTPSPLDGTFCVDSIAALKALEQASSPSGNGSRKSYSLGSIYTRLYWQAPTDSHTAEGDVLTLLSICQWKPQALLQWVDEHARPFSTVKPMYGTPATTGTTNLRPHAATATTPLATANGSPSNGRSRRPKSPPPEKVPEAPSQEGLLAPLSLLTLLTLAIATLYGLFLASPGQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 668
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Major cellular 3'-to-5' DNA exonuclease which digests single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) with mismatched 3' termini. Prevents cell-intrinsic initiation of autoimmunity. Acts by metabolizing DNA fragments from endogenous retroelements, including L1, LTR and SINE elements. Unless degraded, these DNA fragments accumulate in the cytosol and activate the IFN-stimulatory DNA (ISD) response and innate immune signaling. Prevents chronic ATM-dependent checkpoint activation, by processing ssDNA polynucleotide species arising from the processing of aberrant DNA replication intermediates. Inefficiently degrades oxidized DNA, such as that generated upon antimicrobial reactive oxygen production or upon absorption of UV light. During GZMA-mediated cell death, contributes to DNA damage in concert with NME1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.
基因功能參考文獻(xiàn)：
1. Trex1 is an upstream regulator of radiation-driven anti-tumor immunity. Trex1 induction may guide the selection of radiation dose and fractionation in patients treated with immunotherapy. PMID: 28598415
2. the cell-cycle-dependent post-translation modification of TREX1 regulates its interaction with OST. PMID: 28297665
3. The effect of topical TREX1 knockdown and local interferon production on HIV transmission in human cervicovaginal explants and humanized mice, is reported. PMID: 27184854
4. data do not support the concept of retroelement-derived cDNA as key triggers of systemic autoimmunity in Trex1-deficient humans and mice PMID: 28835460
5. Trex1 expression in dendritic cells is essential to prevent breakdown of self-tolerance ensuing from aberrant detection of endogenous DNA. PMID: 27511730
6. Data show that oligosaccharyltransferase (OST) activity is dysregulated in three prime exonuclease 1 knockout (Trex1-/-) cells. PMID: 26320659
7. Data show that cyclic GMP-AMP synthase (cGAS) is essential for all aspects of the autoimmune disease in 3' repair exonuclease Trex1 knockout mice. PMID: 26223655
8. Dysfunctional dsDNA degradation by TREX1 D18N induces disease in mice that recapitulates many characteristics of human lupus. PMID: 25848017
9. knocking out the DNA sensor cyclic GMP-AMP synthase completely abrogates spontaneous induction of IFN-stimulated genes in TREX1-deficient cells. PMID: 24813208
10. Spontaneous type I INF dependent cutaneous pathology in TREX1 deficiency illustrates common pathogenetic pathway in chilblain lupus. PMID: 24270665
11. Upon proinflammatory stimulation, Trex1(-/-) macrophages increase CD86, TNF-alpha & IFN-alpha production, & Ag presentation to CD4(+) T cells, but decrease apoptotic T cell clearance. Trex1 is a negative regulator of macrophage inflammatory activation. PMID: 24218451
12. Oxidized DNA Is less susceptible to TREX1 degradation; the oxidized base 8-hydroxyguanosine, a marker of oxidative damage in DNA, potentiated cytosolic immune recognition by decreasing its susceptibility to 3' repair exonuclease 1 -mediated degradation PMID: 23993650
13. regulates lysosomal biogenesis and interferon-independent activation of antiviral genes PMID: 23160154
14. The structures of the mutant TREX1 proteins provide insight into the dysfunction relating to human disease. PMID: 22071149
15. TREX1 bound to cytosolic HIV DNA and digested excess HIV DNA that would otherwise activate interferon expression via a pathway dependent on the kinase TBK1, the adaptor STING and the transcription factor IRF3 PMID: 20871604
16. Trex1(-/-) mice exhibit a dramatically reduced survival and develop inflammatory myocarditis leading to progressive, often dilated, cardiomyopathy and circulatory failure PMID: 15254239
17. Study defines Trex1 as an essential negative regulator of the IFN-stimulatory DNA response and delineate the genetic pathway linking Trex1 deficiency to lethal autoimmunity. PMID: 18724932
18. Study showed that GFP-TREX1-(1-307) lacking the C-terminal seven amino acids localizes in a perinuclear pattern. PMID: 19442247
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亞細(xì)胞定位：

Nucleus. Cytoplasm, cytosol. Endoplasmic reticulum membrane; Peripheral membrane protein. Note=Retained in the cytoplasm through the C-terminal region. In response to DNA damage, translocates to the nucleus where it is specifically recruited to replication foci. Translocation to the nucleus also occurs during GZMA-mediated cell death.
蛋白家族：

Exonuclease superfamily, TREX family
組織特異性：

Widely expressed with high expression levels detected in spleen, thymus and uterus.
數(shù)據(jù)庫(kù)鏈接：

KEGG: mmu:22040

STRING: 10090.ENSMUSP00000050971

UniGene: Mm.439964