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Recombinant Mouse Steroid 21-hydroxylase (Cyp21)

  • 中文名稱:
    Recombinant Mouse Steroid 21-hydroxylase(Cyp21)
  • 貨號(hào):
    CSB-YP361178MO
  • 規(guī)格:
  • 來(lái)源:
    Yeast
  • 其他:
  • 中文名稱:
    Recombinant Mouse Steroid 21-hydroxylase(Cyp21)
  • 貨號(hào):
    CSB-EP361178MO
  • 規(guī)格:
  • 來(lái)源:
    E.coli
  • 其他:
  • 中文名稱:
    Recombinant Mouse Steroid 21-hydroxylase(Cyp21)
  • 貨號(hào):
    CSB-EP361178MO-B
  • 規(guī)格:
  • 來(lái)源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    Recombinant Mouse Steroid 21-hydroxylase(Cyp21)
  • 貨號(hào):
    CSB-BP361178MO
  • 規(guī)格:
  • 來(lái)源:
    Baculovirus
  • 其他:
  • 中文名稱:
    Recombinant Mouse Steroid 21-hydroxylase(Cyp21)
  • 貨號(hào):
    CSB-MP361178MO
  • 規(guī)格:
  • 來(lái)源:
    Mammalian cell
  • 其他:

產(chǎn)品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    Cyp21
  • Uniprot No.:
  • 別名:
    Cyp21; Cyp21a-1; Cyp21a1; Steroid 21-hydroxylase; EC 1.14.14.16; 21-OHase; Cytochrome P-450c21; Cytochrome P450 21; Cytochrome P450 XXI; Cytochrome P450-C21
  • 種屬:
    Mus musculus (Mouse)
  • 蛋白長(zhǎng)度:
    full length protein
  • 表達(dá)區(qū)域:
    1-487
  • 氨基酸序列
    MLLPGLLLLL LLLAGTRWLW GQWKLRKLHL PPLAPGFLHF LQPNLPIYLL GLTQKLGPIY RIRLGMQDVV VLNSNRTIEE ALIQKWVDFA GRPHMLNGKM DLDLSLGDYS LMWKAHKKLS RSALMLGMRD SMEPLIEQLT QEFCERMRAQ AGTPVAIHKE FSFLTCSIIS CLTFGDKDST LVQTLHDCVQ DLLQAWNHWS IQILTIIPLL RFLPNPGLQK LKQIQESRDH IVKQQLKQHK DSLVAGQWKD MIDYMLQGVE KQRDGKDEER LHEGHVHMSV VDLFIGGTET TATTLSWAVA FLLHHPEIQK RLQEELDLKL GPGSQLLYRN RMQLPLLMAT IAEVLRLRPV VPLALPHRAT RASSISGYDI PKDMVIIPNI QGANLDEMVW ELPSKFWPDR FLEPGKNPRT PSFGCGARVC LGEPLARLEL FVVLARLLQA FTLLPPPDGT LPSLQPQPYA GINLPIPPFQ VRLQPRNLAP QDQGERP
  • 蛋白標(biāo)簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng):
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    A cytochrome P450 monooxygenase that plays a major role in adrenal steroidogenesis. Catalyzes the hydroxylation at C-21 of progesterone and 17alpha-hydroxyprogesterone to respectively form 11-deoxycorticosterone and 11-deoxycortisol, intermediate metabolites in the biosynthetic pathway of mineralocorticoids and glucocorticoids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).
  • 基因功能參考文獻(xiàn):
    1. Cyp21a1 remains expressed in the most distal structure of the developing lung even though these structures are changing, but its expression is not restricted to these areas. PMID: 28216153
    2. Extra-adrenal induction of Cyp21a1 ameliorates steroid metabolism in 21-OHD mice. This study suggests a novel therapeutic strategy for congenital adrenal hyperplasia, which warrants further investigations. PMID: 27432820
    3. Allelic variability for 30-kb deletion in patients with 21-hydroxylase deficiency indicates that a founder effect may be improbable for most monomodular alleles carrying CYP21A1P/A2 chimeric genes in Brazil PMID: 20587039
    4. The mutations are: I, no or minor decrease in enzyme activity: R238Q, P465L, R361K, A362V, P458L; II, loss of enzyme activity caused by inefficient electron flux: R346H, R400C; III, loss of activity due to deficient substrate binding: I462F, L464F PMID: 15731361
  • 亞細(xì)胞定位:
    Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
  • 蛋白家族:
    Cytochrome P450 family
  • 數(shù)據(jù)庫(kù)鏈接:


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