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  4. Recombinant Mouse Single-strand selective monofunctional uracil DNA glycosylase (Smug1)

Recombinant Mouse Single-strand selective monofunctional uracil DNA glycosylase (Smug1)

  • 中文名稱：
    小鼠Smug1重組蛋白
  • 貨號：
    CSB-YP740723MO
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Smug1重組蛋白
  • 貨號：
    CSB-EP740723MO
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Smug1重組蛋白
  • 貨號：
    CSB-EP740723MO-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Smug1重組蛋白
  • 貨號：
    CSB-BP740723MO
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Smug1重組蛋白
  • 貨號：
    CSB-MP740723MO
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Smug1Single-strand selective monofunctional uracil DNA glycosylase; EC 3.2.2.-
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-279
  • 氨基酸序列
    MAASQTFPLG PTHEPASALM EPLPCTRSLA EGFLEEELRL NAELSQLQFP EPVGVIYNPV DYAWEPHRNY VTRYCQGPKE VLFLGMNPGP FGMAQTGVPF GEVNVVRDWL GVGGPVLTPP QEHPKRPVLG LECPQSEVSG ARFWGFFRTL CGQPQVFFRH CFVHNLCPLL FLAPSGRNLT PAELPAKQRE QLLSICDAAL CRQVQLLGVR LVVGVGRLAE QRARRALAGL TPEVQVEGLL HPSPRSAQAN KGWEAAARER LQELGLLPLL TDEGSARPT
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Recognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA substrates. The activity is greater toward mismatches (U/G) compared to matches (U/A). Excises uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA, but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine), nor other oxidized bases. The activity is damage-specific and salt-dependent. The substrate preference is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration, and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration.
  • 基因功能參考文獻：
    1. Nonetheless, using a highly MSH2-dependent mechanism, Ung(-/-) Smug1(-/-) mice can still produce detectable levels of switched isotypes, especially IgG1 PMID: 24771041
    2. SMUG1 is the dominant glycosylase responsible for 5-hydroxymethyluracil-excision in mice as well as the major UNG-backup for U-excision. PMID: 22447450
    3. analysis of species specific differences between mouse and humans in regulation of SMUG1 and UNG2 PMID: 21454529
    4. The structure and specificity of SMUG1 have been solved. PMID: 12820976
  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    Uracil-DNA glycosylase (UDG) superfamily, SMUG1 family
  • 數據庫鏈接：


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