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  4. Recombinant Mouse Ras-related protein Rab-12 (Rab12)

Recombinant Mouse Ras-related protein Rab-12 (Rab12)

  • 中文名稱：
    小鼠Rab12重組蛋白
  • 貨號：
    CSB-YP019160MO
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Rab12重組蛋白
  • 貨號：
    CSB-EP019160MO
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Rab12重組蛋白
  • 貨號：
    CSB-EP019160MO-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Rab12重組蛋白
  • 貨號：
    CSB-BP019160MO
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Rab12重組蛋白
  • 貨號：
    CSB-MP019160MO
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Rab12
  • Uniprot No.：
  • 別名：
    Rab12; Ras-related protein Rab-12; Rab-13
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Full length protein
  • 表達區域：
    1-243
  • 氨基酸序列
    MDPSAALHRR PAGGSLGAVS PALSGGQARR RKQPPRPADF KLQVIIIGSR GVGKTSLMER FTDDTFCEAC KSTVGVDFKI KTVELRGKKI RLQIWDTAGQ ERFNSITSAY YRSAKGIILV YDITKKETFD DLPKWMKMID KYASEDAELL LVGNKLDCET DREISRQQGE KFAQQITGMR FCEASAKDNF NVDEIFLKLV DDILKKMPLD VLRNELSNSI LSLQPEPEIP PELPPPRPHV RCC
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab may play a role in protein transport from recycling endosomes to lysosomes regulating, for instance, the degradation of the transferrin receptor. Involved in autophagy.
  • 基因功能參考文獻：
    1. we identified the small GTPase Rab12 as a novel regulator of mast cell secretory granule transport PMID: 26740112
    2. Dennd3 not only functions as a Rab12-GEF but also modulates Akt signaling in mouse embryonic fibroblast cells PMID: 24719330
    3. A new mechanism of regulation of mTORC1 signalling and autophagy, that is, quality control of PAT4 by Rab12. PMID: 23478338
    4. Knockdown of Rab12 increased transferrin receptor level and reduced M98K-induced cell death. PMID: 23357852
    5. TfR is constitutively degraded by a Rab12-dependent pathway (presumably from recycling endosomes to lysosomes), which is independent of the conventional degradation pathway. PMID: 21718402

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  • 亞細胞定位：
    Recycling endosome membrane; Lipid-anchor; Cytoplasmic side. Lysosome membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane. Cytoplasmic vesicle, autophagosome.
  • 蛋白家族：
    Small GTPase superfamily, Rab family
  • 組織特異性：
    Ubiquitously expressed.
  • 數據庫鏈接：


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