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  4. Recombinant Mouse RNA polymerase-associated protein RTF1 homolog (Rtf1)

Recombinant Mouse RNA polymerase-associated protein RTF1 homolog (Rtf1)

  • 中文名稱：
    小鼠Rtf1重組蛋白
  • 貨號：
    CSB-YP020564MO
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Rtf1重組蛋白
  • 貨號：
    CSB-EP020564MO
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Rtf1重組蛋白
  • 貨號：
    CSB-EP020564MO-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Rtf1重組蛋白
  • 貨號：
    CSB-BP020564MO
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Rtf1重組蛋白
  • 貨號：
    CSB-MP020564MO
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Rtf1
  • Uniprot No.：
  • 別名：
    Rtf1; RNA polymerase-associated protein RTF1 homolog
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Full length protein
  • 表達區域：
    1-715
  • 氨基酸序列
    MRGRLCVGRA AAVAAAVAAA AVAVPLAGGQ EGSQGGVRRG SRGTTMVKKR KGRVVIDSDT EDSGSDENLD QELLSLAKRK RSDSEEKEPP VSQPAASSDS ETSDSDDEWT FGSNKNKKKG KTRKVEKKGA MKKQANKAAS SGSSDRDSSA ESSAPEEGEV SDSESSSSSS SSDSDSSSED EEFHDGYGED LMGDEEDRAR LEQMTEKERE QELFNRIEKR EVLKRRFEIK KKLKTAKKKE KKEKKKKQEE EQEKKKLTQI QESQVTSHNK ERRSKRDEKL DKKSQAMEEL KAEREKRKNR TAELLAKKQP LKTSEVYSDD EEEEDDDKSS EKSDRSSRTS SSDEEEEKEE IPPKSQPVSL PEELNRVRLS RHKLERWCHM PFFAKTVTGC FVRIGIGNHN SKPVYRVAEI TGVVETAKVY QLGGTRTNKG LQLRHGNDQR VFRLEFVSNQ EFTESEFMKW KEAMFSAGMQ LPTLDEINKK ELSIKEALNY KFNDQDIEEI VKEKERFRKA PPNYAMKKTQ LLKEKAMAED LGDQDKAKQI QDQLNELEER AEALDRQRTK NISAISYINQ RNREWNIVES EKALVAESHN MRNQQMDPFT RRQCKPTIVS NSRDPAVQAA ILAQLNAKYG SGVLPDAPKE MSKGQGKDKD LNSKTASDLS EDLFKVHDFD VKIDLQVPSS ESKALAITSK APPAKDGAPR RSLNLEDYKK RRGLI
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both independently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. Binds single-stranded DNA. Required for maximal induction of heat-shock genes. Required for the trimethylation of histone H3 'Lys-4' (H3K4me3) on genes involved in stem cell pluripotency; this function is synergistic with CXXC1 indicative for an involvement of a SET1 complex.
  • 基因功能參考文獻：
    1. Potential role of Rtf1 Plus single stranded DNA binding during transcription elongation. PMID: 18184592
  • 亞細胞定位：
    Nucleus, nucleoplasm.
  • 數據庫鏈接：


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