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  4. Recombinant Mouse RING finger protein 114 (Rnf114)

Recombinant Mouse RING finger protein 114 (Rnf114)

  • 中文名稱：
    小鼠Rnf114重組蛋白
  • 貨號：
    CSB-YP019821MO
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Rnf114重組蛋白
  • 貨號：
    CSB-EP019821MO
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Rnf114重組蛋白
  • 貨號：
    CSB-EP019821MO-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Rnf114重組蛋白
  • 貨號：
    CSB-BP019821MO
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Rnf114重組蛋白
  • 貨號：
    CSB-MP019821MO
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Rnf114
  • Uniprot No.：
  • 別名：
    Rnf114; Zfp228; Zfp313; Znf228; Znf313; E3 ubiquitin-protein ligase RNF114; EC 2.3.2.27; RING finger protein 114; RING-type E3 ubiquitin transferase RNF114; Zinc finger protein 228; Zinc finger protein 313
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-229
  • 氨基酸序列
    MAAAQPESRD GAAQSAKPAS ETDPLSRFTC PVCLEVFEKP VQVPCGHVFC SACLQECLKP KKPVCGVCRS ALAPGVRAVE LERQIESIET SCHGCRKNFI LSKIRAHVTS CSKYQNYIME GVKATTKDAS LQPRNIPNRY TFPCPYCPEK NFDQEGLVEH CKLTHSTDTK SVVCPICASM PWGDPSYRSA NFMEHIQRRH RFSYDTFVDY DVDEDDMINQ VLQRSIIDQ
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    E3 ubiquitin-protein ligase that promotes the ubiquitination of various substrates. In turn, participates in the regulation of many biological processes including cell cycle, apoptosis, osteoclastogenesis as well as innate or adaptive immunity. Acts as negative regulator of NF-kappa-B-dependent transcription by promoting the ubiquitination and stabilization of the NF-kappa-B inhibitor TNFAIP3. May promote the ubiquitination of TRAF6 as well. Acts also as a negative regulator of T-cell activation. Inhibits cellular dsRNA responses and interferon production by targeting MAVS component for proteasomal degradation. Ubiquitinates the CDK inhibitor CDKN1A leading to its degradationand probably also CDKN1B and CDKN1C. This activity stimulates cell cycle G1-to-S phase transition and suppresses cellular senescence. May play a role in spermatogenesis.
  • 基因功能參考文獻：
    1. These data suggested the potential physiological function of RNF114 in inflammation and host antiviral responses, but demonstrate complexity in the regulation of innate immunity by ubiquitin ligases. PMID: 28625874
    2. our study uncovers that RNF114-mediated ubiquitination and degradation of TAB1 activate the NF-kappaB pathway during MZT, and thus directly link maternal clearance to early embryo development. PMID: 28073917
    3. ZNF313 is a novel cell cycle activator with an E3 ligase activity inhibiting cellular senescence by destabilizing p21(WAF1.). PMID: 23645206
    4. Present data suggests that Znf313 gene might play a fundamental role in gene transcription and regulation and relates to spermatogenesis. PMID: 17394778
  • 亞細胞定位：
    Cytoplasm. Nucleus.
  • 數據庫鏈接：


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