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Recombinant Mouse Probable ATP-dependent RNA helicase DDX4 (Ddx4)

  • 中文名稱:
    Recombinant Mouse Probable ATP-dependent RNA helicase DDX4(Ddx4)
  • 貨號:
    CSB-YP717216MO
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    Recombinant Mouse Probable ATP-dependent RNA helicase DDX4(Ddx4)
  • 貨號:
    CSB-EP717216MO
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    Recombinant Mouse Probable ATP-dependent RNA helicase DDX4(Ddx4)
  • 貨號:
    CSB-EP717216MO-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    Recombinant Mouse Probable ATP-dependent RNA helicase DDX4(Ddx4)
  • 貨號:
    CSB-BP717216MO
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    Recombinant Mouse Probable ATP-dependent RNA helicase DDX4(Ddx4)
  • 貨號:
    CSB-MP717216MO
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
  • Uniprot No.:
  • 別名:
    Ddx4; Mvh; VasaATP-dependent RNA helicase DDX4; EC 3.6.4.13; DEAD box protein 4; Mvh; Vasa homolog
  • 種屬:
    Mus musculus (Mouse)
  • 蛋白長度:
    full length protein
  • 表達區域:
    1-702
  • 氨基酸序列
    MGDEDWEAEI LKPHVSSYVP VFEKDKYSSG ANGDTFNRTS ASSDIGESSK KENTSTTGGF GRGKGFGNRG FLNNKFEEGD SSGFWKESNN DCEDNQTRSR GFSKRGGCQD GNDSEASGPF RRGGRGSFRG CRGGFGLGRP NSESDQDQGT QRGGGLFGSR KPAASDSGNG DTYQSRSGSG RGGYKGLNEE VVTGSGKNSW KSETEGGESS DSQGPKVTYI PPPPPEDEDS IFAHYQTGIN FDKYDTILVE VSGHDAPPAI LTFEEANLCQ TLNNNIAKAG YTKLTPVQKY SIPIVLAGRD LMACAQTGSG KTAAFLLPIL AHMMRDGITA SRFKELQEPE CIIVAPTREL INQIYLEARK FSFGTCVRAV VIYGGTQFGH SVRQIVQGCN ILCATPGRLM DIIGKEKIGL KQVKYLVLDE ADRMLDMGFG PEMKKLISCP GMPSKEQRQT LLFSATFPEE IQRLAGDFLK SSYLFVAVGQ VGGACRDVQQ TILQVGQYSK REKLVEILRN IGDERTMVFV ETKKKADFIA TFLCQEKIST TSIHGDREQR EREQALGDFR CGKCPVLVAT SVAARGLDIE NVQHVINFDL PSTIDEYVHR IGRTGRCGNT GRAISFFDTD SDNHLAQPLV KVLSDAQQDV PAWLEEIAFS TYVPPSFSSS TRGGAVFASV DTRKNYQGKH TLNTAGISSS QAPNPVDDES WD
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    ATP-dependent RNA helicase required during spermatogenesis to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle. Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs.
  • 基因功能參考文獻:
    1. BHR also decreased the mRNA level of MVH in the bone marrow, and increased mRNA and protein level of MVH in the ovary o fpremature ovarian failure mice PMID: 27465335
    2. Hgher expression levels of ~55-60 kDa MVH isoform in the ovaries were followed by the accumulations of preovulatory follicles. PMID: 27460133
    3. In mouse oocytes the mitochondrion-originated germinal body-like structures accumulate MVH protein. PMID: 24780413
    4. The results indicate the presence of a developmentally regulated mechanism in which Hat1 and p46 control the physiology of the chromatoid body by acetylation of MVH. PMID: 22223882
    5. Vasa/Ddx4 gene can be involved in regulation of ground and primed states of pluripotency PMID: 22288104
    6. SPSB1 and SPSB4 bind strongly to both Par-4 and VASA peptides. PMID: 20561531
    7. MVH plays crucial roles in the early phase of the ping-pong amplification cycle PMID: 20439430
    8. The RNA helicase Vasa is another essential protein in germline development and arginine methylation is conserved across phyla PMID: 20080973
    9. Data show that RanBPM and mouse vasa homolog (Mvh) are closely associated with perinuclear RNA-protein complexes and chromatoid bodies, and suggests a functional relationship between translational regulation and microtubule nucleation during meiosis. PMID: 14648869

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  • 亞細胞定位:
    Cytoplasm. Cytoplasm, perinuclear region.
  • 蛋白家族:
    DEAD box helicase family, DDX4/VASA subfamily
  • 組織特異性:
    Testis-specific.
  • 數據庫鏈接:


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