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  4. Recombinant Mouse Polycomb group RING finger protein 3 (PcgF3)

Recombinant Mouse Polycomb group RING finger protein 3 (PcgF3)

  • 中文名稱：
    小鼠PcgF3重組蛋白
  • 貨號：
    CSB-YP803934MO
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠PcgF3重組蛋白
  • 貨號：
    CSB-EP803934MO
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠PcgF3重組蛋白
  • 貨號：
    CSB-EP803934MO-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠PcgF3重組蛋白
  • 貨號：
    CSB-BP803934MO
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠PcgF3重組蛋白
  • 貨號：
    CSB-MP803934MO
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    PcgF3; Rnf3; Rnf3aPolycomb group RING finger protein 3; RING finger protein 3A
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-241
  • 氨基酸序列
    MLTRKIKLWD INAHITCRLC SGYLIDATTV TECLHTFCRS CLVKYLEENN TCPTCRIVIH QSHPLQYIGH DRTMQDIVYK LVPGLQEAEM RKQREFYHKL GMEVPGDIKG EACSAKQHLD PRNGETKADD NSNKETAEEK QEEDNDYHRS DEQVSICLEC NSSKLRGLKR KWIRCSAQAT VLHLKKFIAK KLNLSSFNEL DILCNEEILG KDHTLKFVVV TRWRFKKAPL LLHYRPKMDL L
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity. Plays a redundant role with PCGF5 as part of a PRC1-like complex that mediates monoubiquitination of histone H2A 'Lys-119' on the X chromosome and is required for normal silencing of one copy of the X chromosome in XX females.
  • 基因功能參考文獻：
    1. the knockout of Pcgf3, Pcgf5, or both resulted in a 39, 29, and 52% decrease, respectively, in global levels of H2AK119ub1 as compared with wild-type cells, as evidenced by Western blotting. In contrast, no similar change was observed for H3K27me3. PMID: 29054931
    2. Authors conclude that RYBP/YAF2 binding to H2AK119u1 likely underpins the transient interaction of noncanonical PRC1 complexes with Xist RNA domains but does not account for stable interaction of PCGF3/5-PRC1 complexes. PMID: 28596365
    3. hnRNPK recruits PCGF3/5-PRC1 to the Xist RNA B-repeat to establish polycomb-mediated chromosomal silencing. PMID: 29220657
  • 亞細胞定位：
    Nucleus. Nucleus, nucleoplasm.
  • 數(shù)據(jù)庫鏈接：


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