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  4. Recombinant Mouse Phosphoglycerate kinase 2 (Pgk2)

Recombinant Mouse Phosphoglycerate kinase 2 (Pgk2)

  • 中文名稱：
    小鼠Pgk2重組蛋白
  • 貨號：
    CSB-YP017859MO
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Pgk2重組蛋白
  • 貨號：
    CSB-EP017859MO
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Pgk2重組蛋白
  • 貨號：
    CSB-EP017859MO-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Pgk2重組蛋白
  • 貨號：
    CSB-BP017859MO
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Pgk2重組蛋白
  • 貨號：
    CSB-MP017859MO
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Pgk2
  • Uniprot No.：
  • 別名：
    Pgk2; Pgk-2; Phosphoglycerate kinase 2; EC 2.7.2.3; Phosphoglycerate kinase; testis specific
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Full length protein
  • 表達區(qū)域：
    1-417
  • 氨基酸序列
    MALSAKLTLD KVDLKGKRVI MRVDFNVPMK NNQITNNQRI KAAIPSIKHC LDNGAKSVVL MSHLGRPDGI PMPDKYSLEP VADELKSLLN KDVIFLKDCV GPEVEQACAN PDNGSIILLE NLRFHVEEEG KGKDSSGKKI SADPAKVEAF QASLSKLGDV YVNDAFGTAH RAHSSTVGVN LPQKASGFLM KKELDYFSKA LEKPERPFLA ILGGAKVKDK IQLIKNMLDK VNFMIIGGGM AYTFLKELKN MQIGASLFDE EGATIVKEIM EKAEKNGVKI VFPVDFVTGD KFDENAKVGQ ATIESGIPSG WMGLDCGPES IKINAQIVAQ AKLIVWNGPI GVFEWDAFAK GTKALMDEVV KATSNGCVTI IGGGDTATCC AKWGTEDKVS HVSTGGGASL ELLEGKILPG VEALSNM
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Essential for sperm motility and male fertility but is not required for the completion of spermatogenesis.
  • 基因功能參考文獻：
    1. PGK2 is expressed differently in the testes of mouse and pigs according to developmental stage. PMID: 29407897
    2. These results identify multiple signaling and metabolic defects that are likely contributors to male infertility and the absence of progressive sperm motility seen in mice lacking GAPDHS. PMID: 29025010
    3. Data from studies in transgenic mice suggest that Pgk2 core promoter elements are required to direct demethylation of DNA and reconfiguration of nucleosomes in testis during sexual maturation and spermatogenesis. PMID: 24000349
    4. not required for the completion of spermatogenesis, but essential for sperm motility and male fertility PMID: 19759366
    5. PTBP2 is a trans-acting factor that helps to stabilize Pgk2 mRNA in male mouse germ cells. PMID: 17329592
    6. Data suggest that binding of PBX4, plus its coactivator PREP1, is a rate-limiting step leading to the initiation of tissue-specific transcription of the Pgk2 gene. PMID: 17875925
    7. Pgk2 crystal structures have been solved; the apoenzyme is in the "open" form; that is, the N-terminal domain that can bind 3-phosphoglycerate and the C-terminal domain that binds ATP are too far apart for the substrates to interact. PMID: 18004764
    8. t-KH-type splicing regulatory protein binds directly to a 93-nt sequence (designated the F1 region) of the 3'-UTR of the Pgk2 mRNA and destabilizes Pgk2 mRNA constructs in testis extracts. PMID: 19015122

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  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    Phosphoglycerate kinase family
  • 組織特異性：
    Testis and sperm. Localized on the principle piece in the sperm (at protein level). Testis-specific.
  • 數(shù)據(jù)庫鏈接：

    KEGG: mmu:18663

    STRING: 10090.ENSMUSP00000033585

    UniGene: Mm.717



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