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  4. Recombinant Mouse Phosphoethanolamine/phosphocholine phosphatase (Phospho1)

Recombinant Mouse Phosphoethanolamine/phosphocholine phosphatase (Phospho1)

  • 中文名稱：
    小鼠Phospho1重組蛋白
  • 貨號：
    CSB-YP854501MO
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Phospho1重組蛋白
  • 貨號：
    CSB-EP854501MO
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Phospho1重組蛋白
  • 貨號：
    CSB-EP854501MO-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Phospho1重組蛋白
  • 貨號：
    CSB-BP854501MO
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Phospho1重組蛋白
  • 貨號：
    CSB-MP854501MO
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Phospho1
  • Uniprot No.：
  • 別名：
    Phospho1; Phosphoethanolamine/phosphocholine phosphatase; EC 3.1.3.75
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-267
  • 氨基酸序列
    MSGCFPAVGL RCLSRDGRMA APGAPRFLLT FDFDETIVDE NSDDSIVRAA PGQQLPESLR ATYREGYYNE YMQRVFKYLG EQGVRPRDLR AVYETIPLSP GMGDLLQFIA KQGSCFEVIL ISDANTFGVE SALRAAGHHS LFRRILSNPS GPDARGLLTL RPFHTHSCSR CPANMCKHKV LSEYLRERAR DGVHFERLFY VGDGANDFCP MGLLAGGDVA FPRRGYPMHR LIQEAQKAEP SSFRAHVVPW ETAADVRQHL QQVLKMC
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 668
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). Involved in the generation of inorganic phosphate for bone mineralization. Acts in a non-redundant manner with PHOSPHO1 in skeletal mineralization: while PHOSPHO1 mediates the initiation of hydroxyapatite crystallization in the matrix vesicles (MVs), ALPL/TNAP catalyzes the spread of hydroxyapatite crystallization in the extracellular matrix.
  • 基因功能參考文獻：
    1. Osteoclastic resorption of the uncalcified matrix in Phospho1 KO mice was attenuated compared with surrounding normally mineralised bone PMID: 28737011
    2. These data support the involvement of phosphate transporter 1, hereafter referred to as Pi T-1, in the initiation of skeletal mineralization and provide compelling evidence that PHOSPHO1 function is involved in MV biogenesis PMID: 26773408
    3. role in mineralization of alveolar bone and cellular cementum PMID: 27016531
    4. Deficiency in PHOSPHO1 exerts modifications in bone architecture that are transient and depend upon age. PMID: 26232374
    5. ablation of the OPN gene, Spp1, leads to improvements in the skeletal phenotype in Phospho1(-/-) as they age PMID: 24825455
    6. mineralization abnormalities of dentin; reduced overall mineralization with decreased matrix vesicle mineralization in the Phospho1(-/-) mice; almost complete absence of matrix vesicles in mice; further reduction in mineralization. PMID: 23694930
    7. PHOSPHO1 plays a critical role in vascular smooth muscle cell mineralization. PMID: 22887744
    8. Phospho1(-/-) bones are hypomineralized and, consequently, are softer and more flexible. PMID: 21272676
    9. phospho1 has a role in initiation of skeletal calcification PMID: 20684022
    10. The lack of PHOSPHO1 affected ATP hydrolysis via a secondary reduction in the levels of TNAP in PHOSPHO1-deficient matrix vesicles. PMID: 19874193
    11. PHOSPHO1 localization is restricted to mineralizing regions of bone and growth plate PMID: 17227223

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  • 亞細胞定位：
    Extracellular vesicle.
  • 蛋白家族：
    HAD-like hydrolase superfamily, PHOSPHO family
  • 組織特異性：
    Has a 120-fold higher level of expression in bone compared with a range of soft tissues.
  • 數據庫鏈接：


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