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  4. Recombinant Mouse Phosducin-like protein (Pdcl)

Recombinant Mouse Phosducin-like protein (Pdcl)

  • 中文名稱：
    小鼠Pdcl重組蛋白
  • 貨號：
    CSB-YP863584MO
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Pdcl重組蛋白
  • 貨號：
    CSB-EP863584MO
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Pdcl重組蛋白
  • 貨號：
    CSB-EP863584MO-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Pdcl重組蛋白
  • 貨號：
    CSB-BP863584MO
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Pdcl重組蛋白
  • 貨號：
    CSB-MP863584MO
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Pdcl
  • Uniprot No.：
  • 別名：
    Pdcl; PhLP1; Phosducin-like protein; PHLP
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    2-301
  • 氨基酸序列
    TTLDDKLLG EKLQYYYSTS EDEDSDHEDK DRGRGAPAIS STPAEAELAG EGISINTGPK GVINDWRRFK QLETEQREEQ CREMERLIKK LSMSCRSHLD EEEEQQKQKD LQEKISGKMT LKEFGTKDKN LDDEEFLQQY RKQRMEEMRQ QFHKGPQFKQ VFEIPSGEGF LDMIDKEQKS TLIMVHIYED GVPGTEAMNG CMICLATEYP AVKFCRVRSS VIGASSRFTR NALPALLIYK AGELIGNFVR VTDQLGEDFF AVDLEAFLQE FGLLPEKEVL VLTSVRNSAT CHSEDSDLEI D
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Functions as a co-chaperone for CCT in the assembly of heterotrimeric G protein complexes, facilitates the assembly of both Gbeta-Ggamma and RGS-Gbeta5 heterodimers. Acts also as a positive regulator of hedgehog signaling and regulates ciliary function.
  • 基因功能參考文獻：
    1. Pdcl has been implicated as a positive regulator of Hedgehog signaling that may control the trafficking of signaling receptors to primary cilia. PMID: 29290584
    2. findings reveal a common mechanism of Gbetagamma and RGS9-Gbeta5 assembly in rods and cones, highlighting the importance of PhLP1 and CCT-mediated Gbeta complex formation in G protein signaling. PMID: 25659125
    3. evidence of a generic mechanism, whereby the splicing of the PhLP gene could potentially and efficiently regulate the cellular levels of heterotrimeric G proteins. PMID: 23888055
    4. this study demonstrated in vivo that PhLP1 is required for the folding and assembly of both Gbetagamma and Gbeta5-RGS9. PMID: 23637185
    5. These data are consistent with the hypothesis that PhLP is a widely expressed modulator of Gbetagamma function. PMID: 9139665
    6. These results suggest a mechanism for Gbetagamma assembly in which PhLP stabilizes the nascent Gbeta polypeptide until Ggamma can associate, resulting in membrane binding of Gbetagamma and release of PhLP to catalyze another round of assembly. PMID: 15889144
    7. N-glycosylated phosducin-like protein long (PhLP(L)) is expressed in all structures of the central nervous system and regulates opioid receptor function in the brain. PMID: 12015208
    8. identification of novel germ-like specific form in phosducin-like protein family PMID: 12424248
    9. physiological control of G-protein regulation by PhLP seems to involve phosphorylation by CK2 and alternative splicing of the regulator PMID: 12466282

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  • 亞細胞定位：
    Cell projection, cilium.
  • 蛋白家族：
    Phosducin family
  • 數據庫鏈接：


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