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  4. Recombinant Mouse Outer dense fiber protein 2 (Odf2), partial

Recombinant Mouse Outer dense fiber protein 2 (Odf2), partial

  • 中文名稱：
    小鼠Odf2重組蛋白
  • 貨號：
    CSB-YP016272MO
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Odf2重組蛋白
  • 貨號：
    CSB-EP016272MO
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Odf2重組蛋白
  • 貨號：
    CSB-EP016272MO-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Odf2重組蛋白
  • 貨號：
    CSB-BP016272MO
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Odf2重組蛋白
  • 貨號：
    CSB-MP016272MO
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Odf2
  • Uniprot No.：
  • 別名：
    Odf2; Odf84; Outer dense fiber protein 2; 84 kDa outer dense fiber protein; Cenexin; Outer dense fiber of sperm tails protein 2
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Seems to be a major component of sperm tail outer dense fibers (ODF). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail. May have a modulating influence on sperm motility. Functions as a general scaffold protein that is specifically localized at the distal/subdistal appendages of mother centrioles. Component of the centrosome matrix required for the localization of PLK1 and NIN to the centrosomes. Required for the formation and/or maintenance of normal CETN1 assembly.
  • 基因功能參考文獻：
    1. The authors developed an Mrkn2 knockout mouse model to study the role of this gene, and found that deletion of Mkrn2 in mice led to male infertility and expression of Odf2, a vital protein in spermatogenesis, was significantly decreased. PMID: 28008940
    2. the C-terminal region (amino acids 214-638) of Odf2 is required for association with Tssk4; demonstrated the phosphorylation site in Odf2 by Tssk4 PMID: 26961893
    3. Pax6 directly regulates the activity of the Odf2 gene encoding for the appendage-specific protein Odf2 with a role for the assembly of mother centriole. PMID: 25352170
    4. The presence of multiple splicing variants hints that the function of Odf2 is diversified in such a way that each variant has a distinct role in the complex cellular and developmental processes. PMID: 23343771
    5. In adult mice, Odf2 expression was marked in the interdental cells, Boettcher cells, the root cells and root cell processes. Fainter expression was also visible in the hair cells of the maculae and cristae PMID: 22446089
    6. C-terminal region of Odf2 in mice is sufficient for ciliogenesis, the resulting basal bodies lack basal feet; loss of basal feet in ciliated epithelia disrupts polarized organization of apical microtubule lattice without affecting planar cell polarity. PMID: 22265411
    7. Results document the possible impact of loss of one Odf2 allele on sperm tail structure and function, resulting in a novel sperm tail phenotype. PMID: 20550699
    8. Odf2 contributes to assorted ciliopathies PMID: 19756757
    9. Odf2 is indispensable for the formation of distal/subdistal appendages and the generation of primary cilia, but not for other cell-cycle-related centriolar functions. PMID: 15852003
    10. Knockout of the abundant sperm tail protein, outer dense fiber 2, results in preimplantation lethality. PMID: 17078042
    11. ODF2 is the main isoform in testicular tissue RT-PCR analyses revealed that isoforms are not restricted to specific tissues PMID: 18160784
    12. findings show that testicular expression of Odf2 correlates with spermiogenesis and ongoing sperm tail formation thus implicating functional differences between ODF2 and Cenexin. PMID: 18171590

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  • 亞細胞定位：
    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cell projection, cilium. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cytoplasm, cytoskeleton, spindle pole. Cell projection, cilium, flagellum.
  • 蛋白家族：
    ODF2 family
  • 組織特異性：
    Testis-specific (at protein level). Expressed in spermatids at tubular stage V of the spermatogenic cycle. Highly expressed in the cytoplasm of elongating spermatids (tubular stages X/XI). In step 14/15 spermatids of tubular stage III/IV low expression de
  • 數據庫鏈接：


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