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  4. Recombinant Mouse Nucleoside diphosphate kinase, mitochondrial (Nme4)

Recombinant Mouse Nucleoside diphosphate kinase, mitochondrial (Nme4)

  • 中文名稱：
    小鼠Nme4重組蛋白
  • 貨號：
    CSB-YP893429MO
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Nme4重組蛋白
  • 貨號：
    CSB-EP893429MO
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Nme4重組蛋白
  • 貨號：
    CSB-EP893429MO-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Nme4重組蛋白
  • 貨號：
    CSB-BP893429MO
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Nme4重組蛋白
  • 貨號：
    CSB-MP893429MO
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Nme4
  • Uniprot No.：
  • 別名：
    Nme4; Nucleoside diphosphate kinase; mitochondrial; NDK; NDP kinase; mitochondrial; EC 2.7.4.6; Nucleoside diphosphate kinase D; NDPKD; nm23-M4
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    33-186
  • 氨基酸序列
    PWPQERTL VAVKPDGVQR RLVGTVIQRF ERRGFKLVGM KMLQAPESIL AEHYRDLQRK PFYPALISYM SSGPVVAMVW EGPNVVHISR AMIGHTDSTE AAPGTIRGDF SVHISRNVIH ASDSVDGAQR EIELWFQSSE LLNWADGGHH SSCYPA
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Through the catalyzed exchange of gamma-phosphate between di- and triphosphonucleosides participates in regulation of intracellular nucleotide homeostasis. Binds to anionic phospholipids, predominantly to cardiolipin; the binding inhibits its phosphotransfer activity. Acts as mitochondria-specific NDK; its association with cardiolipin-containing mitochondrial inner membrane is coupled to respiration suggesting that ADP locally regenerated in the mitochondrion innermembrane space by its activity is directly taken up via ANT ADP/ATP translocase into the matrix space to stimulate respiratory ATP regeneration. Proposed to increase GTP-loading on dynamin-related GTPase OPA1 in mitochondria. In vitro can induce liposome cross-linking suggesting that it can cross-link inner and outer membranes to form contact sites, and promotes intermembrane migration of anionic phosphoplipids. Promotes the redistribution of cardiolipin between the mitochondrial inner membrane and outer membrane which is implicated in pro-apoptotic signaling.
  • 亞細胞定位：
    Mitochondrion intermembrane space; Peripheral membrane protein. Mitochondrion matrix.
  • 蛋白家族：
    NDK family
  • 組織特異性：
    Expressed in the base region of the oxyntic and pyloric mucosae.
  • 數據庫鏈接：


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