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  4. Recombinant Mouse Nuclear factor 1 X-type (Nfix)

Recombinant Mouse Nuclear factor 1 X-type (Nfix)

  • 中文名稱：
    小鼠Nfix重組蛋白
  • 貨號：
    CSB-YP015758MO
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Nfix重組蛋白
  • 貨號：
    CSB-EP015758MO
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Nfix重組蛋白
  • 貨號：
    CSB-EP015758MO-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Nfix重組蛋白
  • 貨號：
    CSB-BP015758MO
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Nfix重組蛋白
  • 貨號：
    CSB-MP015758MO
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Nfix
  • Uniprot No.：
  • 別名：
    Nfix; Nuclear factor 1 X-type; NF1-X; Nuclear factor 1/X; CCAAT-box-binding transcription factor; CTF; Nuclear factor I/X; NF-I/X; NFI-X; TGGCA-binding protein
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Full length protein
  • 表達區域：
    1-488
  • 氨基酸序列
    MYSPYCLTQD EFHPFIEALL PHVRAFSYTW FNLQARKRKY FKKHEKRMSK DEERAVKDEL LGEKPEIKQK WASRLLAKLR KDIRPEFRED FVLTITGKKP PCCVLSNPDQ KGKIRRIDCL RQADKVWRLD LVMVILFKGI PLESTDGERL YKSPQCSNPG LCVQPHHIGV TIKELDLYLA YFVHTPESGQ SDSSNQQGDA DIKPLPNGHL SFQDCFVTSG VWNVTELVRV SQTPVATASG PNFSLADLES PSYYNINQVT LGRRSITSPP STSSTKRPKS IDDSEMESPV DDVFYPGTGR SPAAGSSQSS GWPNDVDAGP ASLKKSGKLD FCSALSSQGS SPRMAFTHHP LPVLAGVRPG SPRATASALH FPSTSIIQQS SPYFTHPTIR YHHHHGQDSL KEFVQFVCSD GSGQATGQPN GSGQGKVPGS FLLPPPPPVA RPVPLPMPDS KTTSTAPDGA ALTPPSPSFT TTGASSANRF VGIGPRDG
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. Isoform NFIX1 acts as a transcriptional activator while isoform NFIX3 acts as a repressor.
  • 基因功能參考文獻：
    1. This study reveal Foxj1, a key factor promoting ependymal cell maturation, as a target for NFIX-mediated transcriptional activation. PMID: 29452604
    2. overexpression in dystrophic muscles increases regeneration and markedly exacerbates the pathology PMID: 29057908
    3. suggest that NFIX plays a role in regulating progenitor cell biology within the embryonic and post-natal cerebellum, as well as an ongoing role within multiple neuronal and glial populations within the adult cerebellum PMID: 27878595
    4. Under basal conditions, adult hippocampal neural stem cells (AH-NSCs) generate neurons and astrocytes, but not oligodendrocytes. The factors limiting oligodendrocyte production, however, remain unclear. Here, we reveal that the transcription factor NFIX plays a key role in this process. PMID: 29437824
    5. These data demonstrate functional cooperation between Sox6 and Nfix in regulating MyHC-I expression during prenatal muscle development. PMID: 27880909
    6. Nfix deficiency delays intermediate progenitor cell production and prolongs the neurogenic window, resulting in an increased number of neurons in the postnatal forebrain. PMID: 27965439
    7. Nfix is required for maintaining muscle physiology postnatally and for the proper timing of muscle regeneration. PMID: 26923583
    8. Findings suggest that nuclear factor one regulates both proliferation and migration during the development of the subventricular zone neurogenic niche. PMID: 25331604
    9. The data indicate that NFIX influences the lineage specification of postnatal subventricular zone neural stem cells, specifically suppressing oligodendrogenesis. PMID: 26083238
    10. The ependymal layer of the lateral ventricles was frequently absent in Nfix(-/-) mice PMID: 25960350
    11. NFIX acts as a regulator of lineage specification in the haematopoietic system. PMID: 25780920
    12. NFIX plays a central role in hippocampal morphogenesis, regulating the formation of neuronal and glial populations within this PMID: 23042739
    13. Bbx is a downstream target of NFIX during development of the forebrain. PMID: 23852417
    14. The findings demonstrate that the level of Nfix expression during development and within the adult is essential for the function of the hippocampus during learning and memory. PMID: 23776487
    15. Nfix is a novel regulator of murine hematopoietic stem and progenitor cell survival. PMID: 24041575
    16. NFIX is robustly induced when neural stem cells enter quiescence PMID: 23964093
    17. During vertebrate evolution, the transcription factor Nfix lost some specific functions, probably as a consequence of the different environment in which teleosts and mammals develop. PMID: 23482488
    18. The development of cerebellar granule neurons and Purkinje cells within the postnatal cerebellum is delayed in NFIX knock-out mice. PMID: 21800304
    19. Knockout of the transcription factor gene nuclear factor IX (NFIX) in mice produced impaired development of the corpus callosum and severe skeletal defects. PMID: 18983696
    20. Nfix acts as a transcriptional switch from embryonic to fetal myogenesis. PMID: 20178747
    21. a critical role for NFI-X binding in regulating Ren-1 expression PMID: 12591615
    22. Data show that loss of nuclear factor I X is postnatally lethal and leads to hydrocephalus, partial agenesis of the corpus callosum, and deformation of the spine. PMID: 17353270
    23. Nfix is essential for normal brain development and may be required for neural stem cell homeostasis PMID: 18477394
    24. NFIX and HSF1 exert a mutual transcriptional repressive effect on each other which requires CGG repeat in HSF1 promoter and HSF1 binding site in NFIX promoter PMID: 19337383

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    CTF/NF-I family
  • 數據庫鏈接：


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