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  4. Recombinant Mouse Nitrilase homolog 1 (Nit1)

Recombinant Mouse Nitrilase homolog 1 (Nit1)

  • 中文名稱：
    小鼠Nit1重組蛋白
  • 貨號：
    CSB-YP848685MO
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Nit1重組蛋白
  • 貨號：
    CSB-EP848685MO
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Nit1重組蛋白
  • 貨號：
    CSB-EP848685MO-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Nit1重組蛋白
  • 貨號：
    CSB-BP848685MO
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Nit1重組蛋白
  • 貨號：
    CSB-MP848685MO
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Nit1
  • Uniprot No.：
  • 別名：
    Nit1Deaminated glutathione amidase; dGSH amidase; EC 3.5.1.128; Nitrilase homolog 1
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-323
  • 氨基酸序列
    MLGFITRPPH QLLCTGYRLL RTPVLCTQPR PRTMSSSTSW ELPLVAVCQV TSTPNKQENF KTCAELVQEA ARLGACLAFL PEAFDFIARN PAETLLLSEP LNGDLLGQYS QLARECGIWL SLGGFHERGQ DWEQNQKIYN CHVLLNSKGS VVASYRKTHL CDVEIPGQGP MRESNYTKPG GTLEPPVKTP AGKVGLAICY DMRFPELSLK LAQAGAEILT YPSAFGSVTG PAHWEVLLRA RAIESQCYVI AAAQCGRHHE TRASYGHSMV VDPWGTVVAR CSEGPGLCLA RIDLHFLQQM RQHLPVFQHR RPDLYGSLGH PLS
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Catalyzes the hydrolysis of the amide bond in N-(4-oxoglutarate)-L-cysteinylglycine (deaminated glutathione), a metabolite repair reaction to dispose of the harmful deaminated glutathione. Possesses amidase activity toward deaminated ophthalmate in vitro. Plays a role in cell growth and apoptosis: loss of expression promotes cell growth, resistance to DNA damage stress and increased incidence to NMBA-induced tumors. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells.
  • 基因功能參考文獻：
    1. Data show that cisplatin response was enhanced in human lung cancer cells when Nit1 was knocked down and mice Nit1-/-:KrasG12D/+ tumors showed increased sensitivity to cisplatin in vivo. PMID: 26967383
    2. Nit1 and Fhit share tumor suppressor signaling pathways, while localization of the NIT1 gene at a stable chromosome site explains the paucity of gene alterations and in frequent loss of expression of the NIT1 gene in human malignancies. PMID: 16864578
    3. genetic deficiency results in T cell hyperproliferative responses induced by TCR stimulation, spontaneous cell cycle entry and enhanced cell cycle progression PMID: 19395373
    4. the extent of tumor susceptibility due to Nit1 and Fhit deficiency is additive PMID: 19479888
  • 亞細胞定位：
    [Isoform 1]: Mitochondrion.; [Isoform 2]: Cytoplasm.
  • 蛋白家族：
    Carbon-nitrogen hydrolase superfamily, NIT1/NIT2 family
  • 組織特異性：
    Expressed in most tissues with higher expression in adult liver and kidney as well as in fetal adrenal gland and skeletal muscle.
  • 數據庫鏈接：


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