Recombinant Mouse Nephrin (Nphs1), partial

中文名稱：

Recombinant Mouse Nephrin (Nphs1), partial
貨號：

CSB-YP870792MO
規格：

￥1656
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

Nphs1
Uniprot No.：

Q9QZS7
別名：

Renal glomerulus-specific cell adhesion receptor
蛋白長度：

Partial
來源：

Yeast
分子量：

24.2 kDa
表達區域：

36-250aa
氨基酸序列

AQSPVPTSAPRGFWALSENLTVVEGSTVKLWCGVRAPGSVVQWAKDGLLLGPNPKIPGFPRYSLEGDSAKGEFHLLIEACDLSDDAEYECQVGRSELGPELVSPSVILSILVSPKVLQLTPEAGSTVTWVAGQEYVVTCVSGDAKPAPDIIFIQGGRTVEDVSSSVNEGSEEKLFFTEAEARVTPQSSDNGQLLVCEGSNPALATPIKASFTMNI
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

C-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion.
基因功能參考文獻：
1. Nephrin is necessary for podocyte recovery following injury in an adult mature glomerulus PMID: 29924795
2. Here, we found the dynamic changes of Notch1 were coincided with autophagy: they both increased during kidney development and podocyte differentiation...to better understand the relationship between Notch signalling and autophagy in podocyte differentiation, rapamycin was added to enhance autophagy levels in DAPT-treated cells, and as a result, nephrin was recovered and DAPT-induced injury was ameliorated PMID: 28158917
3. WHSC1L1-L acts as a histone methyltransferase in podocytes and regulates nephrin gene expression, which may in turn contribute to the integrity of the slit diaphragm of the glomerular filtration barrier. PMID: 28228401
4. Data (including data from studies using transgenic mice, an murine experimental model of diabetes, and mouse/human cell lines) suggest prostaglandin I2 receptor (Ptgir) is involved in insulin secretion in pancreatic beta-cells and in permselectivity in glomerular podocytes; the mechanism appears to involve regulation of post-translational phosphorylation of nephrin. PMID: 26868296
5. the interaction between nephrin and MAGI-1 regulates Rap1 activation in podocytes to maintain long term slit diaphragm structure PMID: 27707879
6. interaction of full-length suPAR with alphavbeta3 integrin expressed on podocytes results in down-modulation of nephrin that may affect kidney functionality in different human pathologies characterized by increased concentration of suPAR. PMID: 26380915
7. Nphs1 is an activity dependent gene in mouse olfactory sensory neurons. PMID: 24692514
8. Nephrin is involved in pancreatic beta-cell survival signaling; a marked decrease in nephrin expression and phosphorylated Akt was observed in pancreatic islets of leptin receptor-deficient diabetic mice. PMID: 25448064
9. SHP-1 contributes to nephrin deactivation in podocytes exposed to high glucose levels. PMID: 25404734
10. The aPKC-Par3 complex regulates the cell-surface localization of nephrin. PMID: 24700503
11. The planar cell polarity pathway contributes to podocyte development by regulating nephrin turnover during junctional remodeling as the cells differentiate. PMID: 23824190
12. Robo2 signaling acts as a negative regulator on nephrin to influence podocyte foot process architecture. PMID: 22840396
13. Notch signaling induces endocytosis of nephrin, thereby triggering the onset of proteinuria. PMID: 22052054
14. 1,25(OH)(2)D(3) stimulates nephrin expression in podocytes by acting on a VDRE in the proximal nephrin promoter. PMID: 21803771
15. Data show that the main slit diaphragm proteins, nephrin and podocin, are affected from the earlier stages of lupus nephritis and their expression correlates with disease histology. PMID: 21478284
16. Nephrin directly interacts with p75NTR and reveals an important role for nephrin in murine cardiac development by permitting survival of cardiovascular progenitor cells. PMID: 21402589
17. PKC alpha mediates beta-arrestin2-dependent nephrin endocytosis in hyperglycemia. PMID: 21321125
18. PKCalpha is involved in reduction of nephrin surface expression; activation of PKCalpha is a pathomechanistic key event during the development of diabetic nephropathy PMID: 20419132
19. HGF protects podocytes from a loss of nephrin, at least in part, through maintaining synaptopodin in nephritic mice with albuminuria. PMID: 21342325
20. Coexpression of CIN85/Ruk(L) with CD2AP led to a decreased binding of CIN85/Ruk(L) to nephrin and podocin, which indicates a functional competition between CD2AP and CIN85/Ruk(L). PMID: 20457601
21. Expression of nephrin in podocytes is regulated by the cAMP-RAR pathway and suppression of nephrin by TNF-alpha is caused, at least in part, through selective inhibition of this pathway. PMID: 20237236
22. These observations indicate that podocyte-specific expression of rat nephrin can rescue nephrin-deficient mice from perinatal death, but is not sufficient for full complementation. PMID: 19948823
23. Nephrin and Neph3 might regulate certain developmental aspects of Ptf1a-positive neurons as homo- or heterooligomers. PMID: 19887377
24. nephrin and Neph1 interact and have roles in glomerular permeability PMID: 12865409
25. Nphs1 contributes to a porous slit diaphragm scaffold, the molecular filter in renal glomerular capillaries. PMID: 15545998
26. The abnormality of a single podocyte molecule, caused by a single gene mutation in alpha Cd2ap can lead to hereditary nephrotic syndromes. PMID: 16501493
27. Results elucidated the bidirectional regulation of nephrin by pathophysiologic substances and may provide molecular bases for how proteinuria is induced and why some ligands for nuclear receptors have the anti-proteinuric potential. PMID: 16820792
28. Our data suggest that PKCalpha activation may be an important intracellular signaling pathway in the regulation of nephrin expression and glomerular albumin permeability in the diabetic state. PMID: 16955103
29. Expression of nephrin mRNA as well as nephrin promoter activity was suppressed by a putative dedifferentiation factor of podocytes, hepatocyte growth factor. PMID: 17244893
30. Studies in mice have shown that sFlt-1 may play a role in inducing proteinuria by neutralizing vascular endothelial growth factor (VEGF) and suppressing nephrin and synaptopodin. PMID: 17255128
31. Nuclear relocation of nephrin promotes apoptosis of podocytes. PMID: 17537921
32. Nephrin-Neph1 complex transduces phosphorylation-mediated signals that assemble an actin polymerization complex at the podocyte intercellular junction. PMID: 17923684
33. Nephrin does not seem to have a primary role in cell survival but has a small impact on gene regulation during glomerular development. PMID: 18046313
34. Neph1-Nephrin proteins bind the Par3-Par6-atypical protein kinase C (aPKC) complex to regulate podocyte cell polarity PMID: 18562307
35. Results suggest that Kirrel3 plays a role in the axonal pathfinding, cell recognition, and synapse formation of DRG neurons on target cells, including the targeting of proprioceptive neurons on muscle spindles through the interaction with nephrin. PMID: 18752272
36. Results suggest that tensin2 is involved in expression and extension of nephrin, while tensin2 deficiency may result in proteinuria, associated with the loss of slit integrity. PMID: 19129671
37. The aPKC-PAR3 complex associates with the nephrin-podocin complex in podocytes through direct interaction between PAR3 and nephrin, and the kinase activity of aPKC is required for the appropriate distribution of nephrin and podocin in podocytes. PMID: 19142224
38. Nephrin Tyr-1204 phosphorylation triggers the Ca(2+) response in a PLC-gamma1-dependent fashion. PMID: 19179337
39. Nck is required to maintain adult podocytes and phosphotyrosine-based interactions with nephrin may occur in foot processes of resting, mature podocytes. PMID: 19443634
40. These studies suggest that nephrin plays a role in secondary fusion of myoblasts into nascent myotubes, thus establishing a possible functional conservation with Drosophila Sns. PMID: 19470472
41. results suggested selective cooperation of RAR and VDR in the regulation of the nephrin gene PMID: 19474283
42. Nephrin may normally limit NF-kappaB activity in the podocyte. PMID: 19497968
43. In experimental diabetes, MCP-1 was overexpressed within the glomeruli and the absence of MCP-1 reduced both albuminuria and downregulation of nephrin and synaptopodin PMID: 19587356
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亞細胞定位：

Cell membrane; Single-pass type I membrane protein.
蛋白家族：

Immunoglobulin superfamily
組織特異性：

Expressed in kidney glomeruli. In the embryo, expressed in the mesonephric kidney at E11 with strong expression in cranial tubules with podocyte-like structures. Expression is observed in the podocytes of the developing kidney from E13. High expression is
數據庫鏈接：

KEGG: mmu:54631

STRING: 10090.ENSMUSP00000006825

UniGene: Mm.437830