Recombinant Mouse Necdin (Ndn)

中文名稱：

Recombinant Mouse Necdin (Ndn)
貨號：

CSB-CF015606MO
規格：

￥5268
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

Ndn
Uniprot No.：

P25233
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length
來源：

in vitro E.coli expression system
分子量：

44.3 kDa
表達區域：

1-325aa
氨基酸序列

MSEQSKDLSDPNFAAEVPDCEMQDSDAVPVGIPPPASLAANLAGPPCAPEGPMAAQQASPPPEERIEDVDPKILQQAAEEGRAHQPQSPARPIPAPPAPAQLVQKAHELMWYVLVKDQKRMVLWFPDMVKEVMGSYKKWCRSILRRTSVILARVFGLHLRLTNLHTMEFALVKALSPEELDRVALNNRMPMTGLLLMILSLIYVKGRGAREGAVWNVLRILGLRPWKKHSTFGDVRKIITEEFVQQNYLKYQRVPHIEPPEYEFFWGSRANREITKMQIMEFLARVFKKDPQAWPSRYREALEQARALREANLAAQAPRSSVSED
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Growth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle-promoting proteins such as SV40 large T antigen, adenovirus E1A, and the transcription factor E2F. Necdin also interacts with p53 and works in an additive manner to inhibit cell growth. Functions also as transcription factor and binds directly to specific guanosine-rich DNA sequences.
基因功能參考文獻：
1. Overall, these results demonstrate that an increase of SERT activity is sufficient to cause the apneas in Necdin-knockout pups. PMID: 29087295
2. The Necdin expression was further associated with suppression of both cell proliferation and death in osteoblasts. PMID: 28528976
3. Data reveal that necdin promotes mitochondrial biogenesis through stabilization of endogenous PGC-1alpha to exert neuroprotection against mitochondrial insults. PMID: 26971449
4. Our data suggest that necdin suppresses PIAS1 both by inhibiting SUMO E3 ligase activity and by promoting ubiquitin-dependent degradation. PMID: 24911587
5. Necdin is a candidate downstream effector for Galphao. PMID: 25012566
6. Antagonistic interplay between necdin and Bmi1 controls proliferation of neural precursor cells in the embryonic mouse neocortex. PMID: 24392139
7. using several mouse models, we reveal a competition between non-imprinted Ndn promoters which results in monoallelic (paternal or maternal) Ndn expression, suggesting that Ndn allelic exclusion occurs in the absence of imprinting regulation. PMID: 24039599
8. oxygen tension regulates the necdin protein level in NSCs through HIF-2alpha-mediated proteasomal PMID: 23785149
9. necdin exerts its pro-survival activity by counteracting the action of the pro-apoptotic protein Cell Cycle Apoptosis Regulatory Protein (CCAR1/CARP1) PMID: 22905258
10. Necdin-null adult hematopoietic stem cells are less quiescent and more proliferative than normal hematopoietic stem cells, demonstrating the similar role of necdin and p53 in promoting hematopoietic stem cells quiescence during steady-state conditions. PMID: 22776820
11. necdin has multiple roles within protein complexes in different subcellular compartments, and indicate that it can utilize multiple karyopherin-dependent pathways to modulate its localization. PMID: 22442722
12. These results suggest that necdin regulates Foxo1 acetylation and neuropeptide gene expression in the arcuate neurons to modulate the hypothalamic-pituitary-thyroid axis during development. PMID: 22514318
13. Necdin prevents excessive preadipocyte proliferation induced by adipogenic stimulation to control white adipocyte number during adipose tissue development. PMID: 22292082
14. Necdin is a binding partner of dysbindin-1. Dysbindin-1 recruits necdin to the cytoplasm, thereby attenuating the repressive effects of necdin on p53 transcriptional activity. PMID: 21502952
15. Necdin is implicated through the TNF-receptor 1 pathway in the developmental death of motoneuron PMID: 21912643
16. demonstrated that CREB and FoxO1 are recruited to the necdin promoter, likely interacting with specific consensus sequences in the proximal region PMID: 21862615
17. The necdin gene is imprinted, with preferential expression from the paternal allele in human and mouse. PMID: 9302265
18. necdin mediates the terminal differentiation and survival of NGF-dependent dorsal root ganglion neuron and that necdin-deficient nascent neurons are destined to caspase-3-dependent apoptosis. PMID: 12083804
19. Necdin promotes differentiation and survival of neurons through its antagonistic interactions with E2F1. PMID: 12198120
20. Necdin and MAGE-H1 are interactors for the intracellular domain of p75 PMID: 12414813
21. Absence of Ndn causes deficiency of central respiratory drive in newborn mice; most die in the neonatal period of apparent respiratory insufficiency PMID: 12629158
22. The gene expression pattern of Necdin during mouse nervous system development. PMID: 14643685
23. Necdin acts as a master gene regulating smooth muscle (SM) differentiation in at least a subset of SMCs. PMID: 15155529
24. necdin and MAGE-D1 cooperate to modulate the function of Dlx/Msx homeodomain proteins in cellular differentiation PMID: 15272023
25. Data show that NSCL-1 and -2, together with additional cofactors, directly control transcription of the necdin gene. PMID: 15470499
26. Snurf-Snrpn and C/D-box small nucleolar RNAs MBII-85 and MBII-52 and Ndn and Magel2 genes have highly similar expression patterns in the central nervous system, suggesting that they share a common central nervous system-specific regulatory element PMID: 15634360
27. loss of necdin impinges on axonal outgrowth, contributes to the neurological phenotype of Prader-Willi syndrome PMID: 15649943
28. paternally expressed necdin facilitates TrkA signaling to promote the survival of NGF-dependent nociceptive neurons PMID: 16049186
29. These results suggest that paternally expressed necdin facilitates the differentiation and specification of GABAergic neurons in cooperation with Dlx homeodomain proteins. PMID: 16707790
30. Results demonstrate that thyroid hormone regulates the transcription of the Necdin gene. PMID: 16720720
31. These results suggest that endogenous necdin attenuates neuronal apoptosis by suppressing the E2F1-Cdc2 system. PMID: 17108174
32. in early development of the nervous system, Necdin is an anti-apoptotic or survival factor PMID: 17116257
33. These data clarify the function and mechanism of necdin in skeletal muscle and show the importance of necdin in muscle regeneration. PMID: 17954612
34. Knockout mice show variable defects resembling the Prader-Willi syndrome phenotype. PMID: 18085261
35. Ndn-deficiency in mice induces respiratory and serotonin alterations reminiscent of Prader Willi syndrome. PMID: 18085265
36. Lack of Necdin expression induces perinatal serotonergic alterations that affect the maturation and function of the respiratory network, inducing breathing deficits in mice and probably in Prader-Willi patients. PMID: 18272695
37. Results demonstrate a novel role for necdin in cellular migration, in addition to its roles in survival and axon outgrowth. PMID: 18570257
38. These results suggest that necdin downregulates p53 acetylation levels by forming a stable complex with p53 and Sirt1 to protect neurons from DNA damage-induced apoptosis. PMID: 18753379
39. Necdin regulates both the expression of the GnRH gene and GnRH neurons during their development. PMID: 18930956
40. Necdin is expressed in cachectic skeletal muscle to protect fibers from tumor-induced wasting. PMID: 19339547
41. we provide evidence that necdin plays an important role in restricting excessive Hematopoietic stem cell proliferation during hematopoietic regeneration PMID: 19770359
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亞細胞定位：

Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix. Note=Mainly cytoplasmic. Translocates to the nucleus where it is found associated with the nuclear matrix.
組織特異性：

Brain specific. Not detected in other tissues. Expressed in postmitotic neurons. In adult brain the highest expression is in hypothalamus. Highly expressed in thalamus and midbrain. Relatively low levels are in cerebral cortex, hippocampus, striatum, olfa
數據庫鏈接：

KEGG: mmu:17984

STRING: 10090.ENSMUSP00000045369

UniGene: Mm.400253