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  4. Recombinant Mouse NADP-dependent malic enzyme (Me1)

Recombinant Mouse NADP-dependent malic enzyme (Me1)

  • 中文名稱：
    小鼠Me1重組蛋白
  • 貨號：
    CSB-YP013632MO
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Me1重組蛋白
  • 貨號：
    CSB-EP013632MO
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Me1重組蛋白
  • 貨號：
    CSB-EP013632MO-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Me1重組蛋白
  • 貨號：
    CSB-BP013632MO
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Me1重組蛋白
  • 貨號：
    CSB-MP013632MO
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Me1; Mod-1; Mod1NADP-dependent malic enzyme; NADP-ME; EC 1.1.1.40; Malic enzyme 1
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Full length protein
  • 表達(dá)區(qū)域：
    1-572
  • 氨基酸序列
    MEPRAPRRRH THQRGYLLTR DPHLNKDLAF TLEERQQLNI HGLLPPCIIS QELQVLRIIK NFERLNSDFD RYLLLMDLQD RNEKLFYSVL MSDVEKFMPI VYTPTVGLAC QQYSLAFRKP RGLFISIHDK GHIASVLNAW PEDVVKAIVV TDGERILGLG DLGCNGMGIP VGKLALYTAC GGVNPQQCLP ITLDVGTENE ELLKDPLYIG LRHRRVRGPE YDAFLDEFME AASSKYGMNC LIQFEDFANR NAFRLLNKYR NKYCTFNDDI QGTASVAVAG LLAALRITKN KLSDQTVLFQ GAGEAALGIA HLVVMAMEKE GLSKENARKK IWLVDSKGLI VKGRASLTEE KEVFAHEHEE MKNLEAIVQK IKPTALIGVA AIGGAFTEQI LKDMAAFNER PIIFALSNPT SKAECSAEQC YKVTKGRAIF ASGSPFDPVT LPDGRTLFPG QGNNSYVFPG VALGVVACGL RHIDDKVFLT TAEVISQQVS DKHLQEGRLY PPLNTIRGVS LKIAVKIVQD AYKEKMATVY PEPQNKEEFV SSQMYSTNYD QILPDCYPWP AEVQKIQTKV NQ
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點(diǎn)詳情

  • 基因功能參考文獻(xiàn)：
    1. Data suggest that Ad4BP plays role in regulation of intracellular NADPH concentration via transcription of Me1 and Mthfd2 genes in adrenocortical cells. (Ad4BP = nuclear receptor subfamily 5 group A member 1; Me1 = malic enzyme 1; Mthfd2 = bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase) PMID: 28202838
    2. This study showed that (2)H-labeled tracers enable dissection of NADPH production routes across cell types and environmental conditions. PMID: 26999781
    3. ME1 expression was found to be mutant-KRAS-associated in an NSCLC mouse model. PMID: 26173780
    4. p53 represses the expression of the tricarboxylic-acid-cycle-associated malic enzymes ME1 and ME2 in human and mouse cells PMID: 23334421
    5. cytosolic or mitochondrial isoforms of malic enzyme do not affect glucose-stimulated insulin secretion from rodent islets PMID: 18755687
    6. although ME1 overexpression augments anaplerosis and glucose stimulated insulin secretion in INS-1 832/13 cells, it is not likely involved in methyl succinate and glucose stimulated insulin secretion in pancreatic islets PMID: 19293334

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  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    Malic enzymes family
  • 數(shù)據(jù)庫鏈接：


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