Recombinant Mouse Myristoylated alanine-rich C-kinase substrate (Marcks)

中文名稱：

Recombinant Mouse Myristoylated alanine-rich C-kinase substrate (Marcks)
貨號(hào)：

CSB-MP013493MO
規(guī)格：

￥1536
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

Marcks
Uniprot No.：

P26645
別名：

MARCKS
蛋白長(zhǎng)度：

Full Length
來源：

Mammalian cell
分子量：

58.4 kDa
表達(dá)區(qū)域：

2-309aa
氨基酸序列

GAQFSKTAAKGEATAERPGEAAVASSPSKANGQENGHVKVNGDASPAAAEPGAKEELQANGSAPAADKEEPASGSAATPAAAEKDEAAAATEPGAGAADKEAAEAEPAEPSSPAAEAEGASASSTSSPKAEDGAAPSPSSETPKKKKKRFSFKKSFKLSGFSFKKSKKESGEGAEAEGATAEGAKDEAAAAAGGEGAAAPGEQAGGAGAEGAAGGEPREAEAAEPEQPEQPEQPAAEEPQAEEQSEAAGEKAEEPAPGATAGDASSAAGPEQEAPAATDEAAASAAPAASPEPQPECSPEAPPAPTAE
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

C-terminal hFc1-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
基因功能參考文獻(xiàn)：
1. this work establishes a novel role for MARCKS in axon dynamics and highlights the necessity of MARCKS as an organizer of DCC signaling at the membrane. PMID: 29546593
2. Strongly support the existence of a PKC-MARCKS-PI3K regulatory module. PMID: 29715315
3. ENaC activity is regulated by calpain-2 proteolysis of MARCKS. PMID: 28468944
4. In the absence of Pin1, MARCKS is hyper-phosphorylated, leading to loss of cell adhesions, and collapse of the growth cone. PMID: 26991250
5. Findings suggest that MIR429 modulates mucin secretion in human colorectal cells and mouse colitis tissues by up-regulating of MARCKS expression. PMID: 26818658
6. Conditional deletion of MARCKS in ECs induces intracellular accumulation of mucins, elevated oxidative stress, and lipid droplet buildup. PMID: 26010231
7. MARCKS acts as a "molecular switch," binding to and regulating PIP2 signaling to regulate processes like proplatelet extension (microtubule-driven) vs proplatelet branching (Arp2/3 and actin polymerization-driven). PMID: 26744461
8. MARCKS knockdown arrested VSMC cell cycle by decreasing KIS expression. Decreased KIS expression resulted in nuclear trapping of p27kip1 in VSMCs. PMID: 26528715
9. MARCKS regulates the expression of proinflammatory cytokines in macrophages through activation of p38/JNK MAPK and NF-kappaB. PMID: 25929183
10. Targeting phospho-MARCKS overcomes drug-resistance and induces antitumor activity in preclinical models of multiple myeloma. PMID: 25179733
11. Grin 1 is identified as a novel Cdk5 substrate and MARCKS is confirmed as a Cdk5 substrate. PMID: 24658276
12. MARCKS appears to be a nonessential regulatory protein in mast cell exocytosis but exerts a negative modulation. PMID: 24705720
13. MARCKS overexpression in the hippocampus of old mice leads to long-term potentiation and improved memory. PMID: 23434911
14. an important functional role for the interaction between MARCKS and PSA in the developing and adult nervous system. PMID: 23329829
15. cathepsin K exocytosis is controlled by PKCdelta through modulation of the actin bundling protein myristoylated alanine-rich C-kinase substrate. PMID: 22806935
16. These results suggest that MARCKS is involved in directed migration of macrophages PMID: 22623357
17. a critical role for H(2)O(2) in angiotensin-II signaling to the endothelial cytoskeleton in a novel pathway that is critically dependent on MARCKS, Rac1, and c-Abl. PMID: 22773836
18. the capacity of MARCKS-ED to regulate granule exocytosis in a PKC-dependent manner, consistent with regulated sequestration of phosphoinositides that mediate granule fusion at the plasma membrane. PMID: 22013076
19. MARCKS contributes to the negative regulation of the cellular response to LPS. PMID: 22427633
20. L-PGDS protein regulates glial cell migration and morphology through Marcks in prostaglandin D2 independent manner. PMID: 22275363
21. findings suggest that abnormal expression of MARCKS via dysfunction of Dysbindin might cause impairment of neural transmission and abnormal synaptogenesis PMID: 20098743
22. Motif analyses revealed ca. 12 overlapping potential transcription factor binding sites; synergistic interaction between distal and proximal promoter sequences is indispensable for the optimal MARCKS promoter function PMID: 11944910
23. Sequential activation of individual PKC isozymes in integrin-mediated muscle cell spreading: a role for MARCKS in an integrin signaling pathway. PMID: 11973356
24. Hippocampal, total MARCKS mRNA and protein levels in C57BL/6J mice were significantly lower compared with DBA/2J mice; Restraint stress resulted in significant reduction in membrane MARCKS expression in C57BL/6J hippocampus but not for DBA/2J mice PMID: 12675922
25. post-translational modifications of MARCKS are necessary and sufficient to regulate actin-binding activity PMID: 15640140
26. Results show that phosphorylated MARCKS is a novel centrosome component and also defines a previously unrecognized subdomain of the actin cap overlying the spindle. PMID: 15766745
27. Antisense strategy directed against MARCKS, a calpain substrate during C2C12 migration, showed that this protein could play a role in stress fiber polymerization. PMID: 16496301
28. Increased cleavage of MARCKS in brain and macrophages, but not in liver and kidney extracts of stefin B-deficient mice compared to wild-type mice, was observed. PMID: 17655504
29. Data demonstrate in mice that the myristoylated alanine-rich C-kinase substrate protein (MARCKS), a prominent cellular substrate for PKC, modulates radial glial placement and expansion. PMID: 19666823
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亞細(xì)胞定位：

Cytoplasm, cytoskeleton. Membrane; Lipid-anchor.
蛋白家族：

MARCKS family
組織特異性：

Brain, spleen, less in kidney and heart, and very low levels in liver.
數(shù)據(jù)庫(kù)鏈接：

KEGG: mmu:17118

STRING: 10090.ENSMUSP00000090245

UniGene: Mm.30059