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  4. Recombinant Mouse Mitogen-activated protein kinase kinase kinase kinase 1 (Map4k1), partial

Recombinant Mouse Mitogen-activated protein kinase kinase kinase kinase 1 (Map4k1), partial

  • 中文名稱：
    小鼠Map4k1重組蛋白
  • 貨號：
    CSB-YP013436MO
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Map4k1重組蛋白
  • 貨號：
    CSB-EP013436MO
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Map4k1重組蛋白
  • 貨號：
    CSB-EP013436MO-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Map4k1重組蛋白
  • 貨號：
    CSB-BP013436MO
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Map4k1重組蛋白
  • 貨號：
    CSB-MP013436MO
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Map4k1; Hpk1Mitogen-activated protein kinase kinase kinase kinase 1; EC 2.7.11.1; Hematopoietic progenitor kinase; HPK; MAPK/ERK kinase kinase kinase 1; MEK kinase kinase 1; MEKKK 1
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

問答及客戶評論

■ 常見問題解答
Q:

I want to know mouse HPK1 sequence, tag and activity, did you test the enzymatic activity for MAP4K1(HPK1)?

A:
Regarding your inquiry about mouse HPK1, please check information as follows:
Recombinant Mouse Mitogen-activated protein kinase kinase kinase kinase 1(Map4k1) ,partial
CSB-BP013436MO >> Baculovirus
Expression Region: 15-280aa; Partial.
Tag information: Tag type will be determined during the manufacturing process.
We can try your desired tag preferentially if you have, but as we can't 100% guaranteed, if it's failed, we will provide the protein with other tag. The success rate of expressing the protein with your desired tag is 95.25%.
Target protein sequence: (The whole complete sequence will include tag sequene, target protein sequence and any linker sequence)

EHYDLLQRLGGGTYGEVFKARDKVSKDLVALKMVKMEPDDDVATLQKEILMLKTCRHANIVAYHGSYLWLQKLWICMEFCGAGSLQDIYQVTGSLSELQISYVCREVLQGLAYLHSEKKIHRDIKGANILINDCGEVKLADFGISAQIGATLARRLSFIGTPYWMAPEVAAVALKGGYNELCDIWSLGITAIELAELQPPLFDVHPLRVLFLMTKSGYQPPRLKEKSRWSSSFHNFVKVTLTKNSKKRPSATKMLSHQLVSQPGLN


As for the activity, sorry, we haven't detected the activity of this protein, we can't 100% guarantee its activity.
The protein that we provide contain a kinase domain and it's purified under a very mild condition, in theory, it should be active.
According to our experience on expression of kinases, some recombinant fragmens with only kinase domain are enzymatic active, such as
human MAP4K1 (http://www.abcam.cn/active-human-map4k1-protein-fragment-ab107953.html)
human MAPK1 (http://emboj.embopress.org/content/embojnl/21/19/5151.full.pdf)
To our knowledge, the kinases from that of STE Ser/Thr protein kinase family, TKL Ser/Thr protein kinase family and Tyrosine-protein kinase family could be expressed with kinase domain.

靶點詳情

  • 功能：
    Serine/threonine-protein kinase, which may play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. Able to autophosphorylate. Together with CLNK, it enhances CD3-triggered activation of T-cells and subsequent IL2 production.
  • 基因功能參考文獻：
    1. Introduction of three 4-R-hydroxyproline residues stabilizes the SH3m-cortactin binding of HPK1 peptide. PMID: 22976002
    2. a novel negative feedback regulation of BCR signaling by HPK1-mediated phosphorylation, ubiquitination, and subsequent degradation of the activated BLNK PMID: 22334673
    3. HPK1 associates with SKAP1 to negatively regulate Rap1-mediated B-lymphocyte adhesion. PMID: 20824186
    4. Hpk1 supports apoptosis of T lymphocytes by inhibiting the antiapoptotic action of NF-kappaB and inducing the proapoptotic activity of JNK. PMID: 12130508
    5. Mona/Gads SH3C binding to hematopoietic progenitor kinase 1 (HPK1) combines an atypical SH3 binding motif, R/KXXK, with a classical PXXP motif embedded in a polyproline type II (PPII) helix PMID: 15100220
    6. suppression or activation of NFkappaB by HPK1 determines sensitivity to activation-induced cell death PMID: 16341093
    7. HPK1-C as a suppressor of antiapoptotic Bcl-2 proteins and provide a molecular basis for our understanding of CD95L-independent activation-induced cell death of lymphocytes. PMID: 17712048
    8. Our data reveal a novel role for HPK1 as a negative regulator of dendritic cell functions PMID: 19414772

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  • 蛋白家族：
    Protein kinase superfamily, STE Ser/Thr protein kinase family, STE20 subfamily
  • 組織特異性：
    Expressed in hemopoietic cells (at protein level). Ubiquitously expressed in all tissues examined at embryonic stage E16.5 with high levels in lung, heart and fetal liver. In the neonate, expression is restricted to the tissues which undergo lineage decis
  • 數據庫鏈接：


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