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  4. Recombinant Mouse Methyltransferase-like protein 4 (Mettl4)

Recombinant Mouse Methyltransferase-like protein 4 (Mettl4)

  • 中文名稱：
    小鼠Mettl4重組蛋白
  • 貨號：
    CSB-YP666237MO
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Mettl4重組蛋白
  • 貨號：
    CSB-EP666237MO
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Mettl4重組蛋白
  • 貨號：
    CSB-EP666237MO-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Mettl4重組蛋白
  • 貨號：
    CSB-BP666237MO
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Mettl4重組蛋白
  • 貨號：
    CSB-MP666237MO
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Mettl4
  • Uniprot No.：
  • 別名：
    Mettl4N(6)-adenine-specific DNA methyltransferase METTL4; EC 2.1.1.72; Methyltransferase-like protein 4
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-471
  • 氨基酸序列
    MSVVHHLPPG WLLDHLSFIN KVNYQLCQHQ ESFCSKNNPT SSVYMDSLQL DPGSPFGAPA MCFAPDFTTV SGNDDEGSCE VITEKYVFRS ELFNVTKPYI VPAVHKERQQ SNKNENLVTD YKQEVSVSVG KKRKRCIAFN QGELDAMEYH TKIRELILDG SSKLIQEGLR SGFLYPLVEK QDGSSGCITL PLDACNLSEL CEMAKHLPSL NEMELQTLQL MGDDVSVIEL DLSSQIIENN SSFSKMITLM GQKYLLPPQS SFLLSDISCM QPLLNCGKTF DAIVIDPPWE NKSVKRSNRY SSLSPQQIKR MPIPKLAAAD CLIVTWVTNR QKHLCFVKEE LYPSWSVEVV AEWYWVKITN SGEFVFPLDS PHKKPYECLV LGRVKEKTPL ALRNPDVRIP PVPDQKLIVS VPCVLHSHKP PLTEVLRDYI KPGGQCLELF ARNLQPGWMS WGNEVLKFQH MDYFIALESG C
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    N(6)-adenine-specific methyltransferase that can methylate both RNAs and DNA. Acts as a N(6)-adenine-specific RNA methyltransferase by catalyzing formation of N6,2'-O-dimethyladenosine (m6A(m)) on internal positions of U2 small nuclear RNA (snRNA): methylates the 6th position of adenine residues with a pre-deposited 2'-O-methylation. Internal m6A(m) methylation of snRNAs regulates RNA splicing. Also able to act as a N(6)-adenine-specific DNA methyltransferase by mediating methylation of DNA on the 6th position of adenine (N(6)-methyladenosine). The existence of N(6)-methyladenosine (m6A) on DNA is however unclear in mammals, and additional evidences are required to confirm the role of the N(6)-adenine-specific DNA methyltransferase activity of METTL4 in vivo. Acts as a regulator of mitochondrial transcript levels and mitochondrial DNA (mtDNA) copy number by mediating mtDNA N(6)-methylation: m6A on mtDNA reduces transcription by repressing TFAM DNA-binding and bending. N(6)-methyladenosine deposition by METTL4 regulates Polycomb silencing by triggering ubiquitination and degradation of sensor proteins ASXL1 and MPND, leading to inactivation of the PR-DUB complex and subsequent preservation of Polycomb silencing.
  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    MT-A70-like family
  • 數據庫鏈接：


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