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  4. Recombinant Mouse Melanoma-derived growth regulatory protein (Mia)

Recombinant Mouse Melanoma-derived growth regulatory protein (Mia)

  • 中文名稱：
    小鼠Mia重組蛋白
  • 貨號：
    CSB-YP720265MO
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Mia重組蛋白
  • 貨號：
    CSB-EP720265MO
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Mia重組蛋白
  • 貨號：
    CSB-EP720265MO-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Mia重組蛋白
  • 貨號：
    CSB-BP720265MO
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Mia重組蛋白
  • 貨號：
    CSB-MP720265MO
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Mia; Cdrap; Mia1; Melanoma-derived growth regulatory protein; Cartilage-derived retinoic acid-sensitive protein; CD-RAP; Melanoma inhibitory activity protein
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    23-130
  • 氨基酸序列
    DRAMPKLA DWKLCADEEC SHPISMAVAL QDYVAPDCRF LTIYRGQVVY VFSKLKGRGR LFWGGSVQGG YYGDLAARLG YFPSSIVRED LTLKPGKIDM KTDQWDFYCQ
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.
  • 基因功能參考文獻：
    1. The effects of MIA/CD-RAP on transcriptional regulation in chondrocytes, through the regulation of p54(nrb) via YBX1 contributes to the understanding of chondrogenesis. PMID: 24349210
    2. Attenuated LM expressing MIA. PMID: 22886976
    3. as observed in other knockout models of molecules important for cartilage development and differentiation, viability and functional integrity is reached by remarkable molecular redundancy in MIA/CD-RAP knockout mice. PMID: 24269712
    4. The cartilage protein melanoma inhibitory activity contributes to inflammatory arthritis. PMID: 24287514
    5. Expression analysis of cartilage tissue derived from MIA-/- mice revealed strong downregulation of nuclear RNA-binding protein 54-kDa. PMID: 21368873
    6. MIA/CD-RAP stabilizes cartilage differentiation and inhibits differentiation into bone potentially by regulating signaling processes during differentiation. PMID: 20164682
    7. transcriptional mechanism by which CD-RAP expression is suppressed by IL-1 beta PMID: 12072435
    8. Cdrap/Mia is located between two housekeeping genes PMID: 15028289
    9. DNA promoter segment from -2,251 bp to -2,068 bp of the CD-RAP gene contains elements critical for gene expression. It demonstrates activation or repression of gene expression in vitro and in vivo based on cell type and content of transcription factors. PMID: 16250001
    10. A potential tumor suppressor role of Mia/Cd-rap in pituitary cells. PMID: 17914116
    11. Microarray analysis showed significant reductions in the expression of several proteins in lung misexpressing MIA. PMID: 18342301

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    MIA/OTOR family
  • 組織特異性：
    All malignant melanoma cell lines tested and infrequently in glioma cell lines.
  • 數據庫鏈接：

    KEGG: mmu:12587

    UniGene: Mm.262447



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