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  4. Recombinant Mouse Heparan sulfate glucosamine 3-O-sulfotransferase 1 (Hs3st1)

Recombinant Mouse Heparan sulfate glucosamine 3-O-sulfotransferase 1 (Hs3st1)

  • 中文名稱：
    小鼠Hs3st1重組蛋白
  • 貨號：
    CSB-YP010747MO
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Hs3st1重組蛋白
  • 貨號：
    CSB-EP010747MO
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Hs3st1重組蛋白
  • 貨號：
    CSB-EP010747MO-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Hs3st1重組蛋白
  • 貨號：
    CSB-BP010747MO
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Hs3st1重組蛋白
  • 貨號：
    CSB-MP010747MO
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Hs3st1
  • Uniprot No.：
  • 別名：
    Hs3st1; 3ost; 3ost1Heparan sulfate glucosamine 3-O-sulfotransferase 1; EC 2.8.2.23; Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1; Heparan sulfate 3-O-sulfotransferase 1
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Full length protein
  • 表達區域：
    21-311
  • 氨基酸序列
    HPAAPGPGLK QQELLRKVII LPEDTGEGTA SNGSTQQLPQ TIIIGVRKGG TRALLEMLSL HPDVAAAENE VHFFDWEEHY SQGLGWYLTQ MPFSSPHQLT VEKTPAYFTS PKVPERIHSM NPTIRLLLIL RDPSERVLSD YTQVLYNHLQ KHKPYPPIED LLMRDGRLNL DYKALNRSLY HAHMLNWLRF FPLGHIHIVD GDRLIRDPFP EIQKVERFLK LSPQINASNF YFNKTKGFYC LRDSGKDRCL HESKGRAHPQ VDPKLLDKLH EYFHEPNKKF FKLVGRTFDW H
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. Catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site.
  • 基因功能參考文獻：
    1. The expression of heparan sulfate sulfotransferases was examined by in situ hybridization. PMID: 25325966
    2. the 3-OST-1 enzyme produces the majority of tissue anticoagulant heparan sulfate PMID: 12671048
    3. Hs3st1(-/-) mice do not show an obvious procoagulant phenotype. PMID: 12975616
    4. Crystal structure of the binary complex of 3-OST-1 and PAP provide information essential for understanding the biosynthesis of anticoagulant heparan sulfate and the general mechanism of action of heparan sulfate sulfotransferases. PMID: 15060080
    5. In this paper a conformational change is described that occurs in heparan sulfate 3-O-sulfotransferase-1 upon binding to heparan sulfate. PMID: 15096036

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  • 亞細胞定位：
    Golgi apparatus lumen.
  • 蛋白家族：
    Sulfotransferase 1 family
  • 數據庫鏈接：


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