Recombinant Mouse Guanine nucleotide-binding protein G (o) subunit alpha (Gnao1)

中文名稱：

小鼠Gnao1重組蛋白
貨號：

CSB-YP009593MO
規(guī)格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

Gnao1
Uniprot No.：

P18872
別名：

Gnao1; Gna0; GnaoGuanine nucleotide-binding protein G(o) subunit alpha
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length of Mature Protein
來源：

Yeast
分子量：

42.0kDa
表達區(qū)域：

2-354aa
氨基酸序列

GCTLSAEERAALERSKAIEKNLKEDGISAAKDVKLLLLGAGESGKSTIVKQMKIIHEDGFSGEDVKQYKPVVYSNTIQSLAAIVRAMDTLGVEYGDKERKTDSKMVCDVVSRMEDTEPFSAELLSAMMRLWGDSGIQECFNRSREYQLNDSAKYYLDSLDRIGAGDYQPTEQDILRTRVKTTGIVETHFTFKNLHFRLFDVGGQRSERKKWIHCFEDVTAIIFCVALSGYDQVLHEDETTNRMHESLMLFDSICNNKFFIDTSIILFLNKKDLFGEKIKKSPLTICFPEYPGSNTYEDAAAYIQTQFESKNRSPNKEIYCHMTCATDTNNIQVVFDAVTDIIIANNLRGCGLY
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Stimulated by RGS14. The G(o) protein function is not clear.
基因功能參考文獻：
1. Galphao +/- mice developed tolerance faster and displayed more severe naltrexone-precipitated withdrawal in some behaviors than did wild-type PMID: 28971229
2. Go signaling is essential for the structural and functional integrity of the main olfactory epithelium and for specification of olfactory bulb interneurons. PMID: 27625425
3. Data obtained support the hypothesis that GalphaO-mediated signaling within the presympathetic circuits of the rostral ventral lateral medulla contributes to the autonomic control of the heart. PMID: 27528004
4. Necdin is a candidate downstream effector for Galphao. PMID: 25012566
5. This heterozygous Gnao1 +/G184S mutation produces early neonatal and adult lethality accompanied by epileptiform discharges. PMID: 24700286
6. These results highlight that sensory neurons of the Galphao-expressing vomeronasal subsystem, together with the receptors they express and the molecular cues they detect, control a wide range of fundamental mating and reproductive behaviors in female mice. PMID: 24886577
7. The Goalpha expressing vomeronasal receptor cells led to all areas of the accessory olfactory bulb (AOB). PMID: 22383629
8. Data indicate that rectifying potassium channel Kir2.4 interacts with G-protein subunit Galphao. PMID: 23339194
9. Data show that Galphao is an essential requirement for the display of male-male territorial aggression as well as maternal aggression. PMID: 21768373
10. Data suggest that the novel R234H mutation imparts oncogenic properties to Galphao by accelerating nucleotide exchange and rendering it constitutively active. PMID: 21317923
11. The 2.9 A crystal structure of the enigmatic, neuronal G protein Galpha(o) in the GTP hydrolytic transition state, complexed with RGS16. PMID: 18434540
12. Go alpha subunit mainly appears to be bound to the cell membrane and concentrated in the thin finger-like dendrites of rod bipolar cells, as well as in green fluorescent protein-positive cone bipolar cells. PMID: 12454992
13. Binding of activated Galphao to GRIN1 induces activation of Cdc42, which leads to morphological changes in neuronal cells. PMID: 15585744
14. our findings establish an essential role for G(o) in the antiadrenergic effect of muscarinic agent on hyperpolarization current PMID: 18156202
15. Go2alpha optimizes vesicular filling which is instrumental for normal dopamine functioning and for the development of drug-induced behavioral sensitization. PMID: 18606864
16. Data show that Galphao colocalized with GRIN1 at neuronal dendrites and axons, and Galphao-GRIN1 pathway could mediate roles in neuronal migration and differentiation at embryonic stages. PMID: 18729205
17. Report role of Galpha0 in the control of in vivo heart rate dynamics. PMID: 18832081
18. Regulator of G protein signaling protein suppression of Galphao protein-mediated alpha2A adrenergic receptor inhibition of mouse hippocampal CA3 epileptiform activity. PMID: 19225179
19. The G alpha(o) protein in the locus coeruleus as contributing to interindividual variability in physical dependence on opioids in mice. PMID: 19460419
20. Data indicate that MC4R mutant is able to selectively interact with G(i/o) proteins. PMID: 19648111
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亞細胞定位：

Cell membrane. Membrane; Lipid-anchor.
蛋白家族：

G-alpha family, G(i/o/t/z) subfamily
數(shù)據(jù)庫鏈接：

KEGG: mmu:14681

STRING: 10090.ENSMUSP00000034198

UniGene: Mm.251445