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  4. Recombinant Mouse Guanidinoacetate N-methyltransferase (Gamt)

Recombinant Mouse Guanidinoacetate N-methyltransferase (Gamt)

  • 中文名稱：
    小鼠Gamt重組蛋白
  • 貨號(hào)：
    CSB-YP009227MO
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Gamt重組蛋白
  • 貨號(hào)：
    CSB-EP009227MO
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Gamt重組蛋白
  • 貨號(hào)：
    CSB-EP009227MO-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Gamt重組蛋白
  • 貨號(hào)：
    CSB-BP009227MO
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Gamt重組蛋白
  • 貨號(hào)：
    CSB-MP009227MO
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Gamt
  • Uniprot No.：
  • 別名：
    Gamt; Guanidinoacetate N-methyltransferase; EC 2.1.1.2
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Full length protein
  • 表達(dá)區(qū)域：
    1-236
  • 氨基酸序列
    MSSSAASPLF APGEDCGPAW RAAPAAYDAS DTHLQILGKP VMERWETPYM HALAAAAASR GGRVLEVGFG MAIAASRVQQ APIEEHWIIE CNDGVFQRLQ DWALRQPHKV VPLKGLWEEV APTLPDGHFD GILYDTYPLS EEAWHTHQFN FIKNHAFRLL KTGGVLTYCN LTSWGELMKS KYTDITTMFE ETQVPALQEA GFLKENICTE VMALVPPADC RYYAFPQMIT PLVTKH
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Converts guanidinoacetate to creatine, using S-adenosylmethionine as the methyl donor. Important in nervous system development.
  • 基因功能參考文獻(xiàn)：
    1. Inactivation of the creatine kinase system by GAMT deficiency does not alter mitochondrial organization and intracellular compartmentation in relaxed cardiomyocytes. PMID: 23792673
    2. GAMT was expressed highly in oligodendrocytes and olfactory ensheathing glia and moderately in astrocytes, whereas GAMT was very low in neurons and microglia. PMID: 15245487
    3. despite the absence of Cr, GAMT-/- mice can cope with mild ischaemic stress by using phosphorylated guanidinoacetate for high energy phosphoryl transfer PMID: 15284341
    4. GAMT(Cr)-/- mice showed differences in maximal rates of force rise and relaxation times during the isometric protocol as well as in force during the dynamic protocol. PMID: 15743892
    5. Our results can provide the basis for additional experiments to optimize Cr supplementation in GAMT deficiency, as increases in brain Cr are slow in patients after Cr supplementation. PMID: 17347380
    6. GAMT and AGAT mRNA are up-regulated 5.4- and 1.9-fold respectively in adult mdx muscle compared to C57 PMID: 17588756
    7. In the fetal brain, mRNA expression of GAMT was relatively low until 34 days gestation (term is 38-39 days). In the fetal kidney and liver, GAMT mRNA and protein expression were also relatively low until 34-37 days gestation. PMID: 19570237

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  • 亞細(xì)胞定位：
    Cell projection, microvillus. Note=Detected in microvilli of the epithelial cells lining the caput epididymis.
  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, RMT2 methyltransferase family
  • 組織特異性：
    Highly expressed in testis, caput epididymis, ovary, and liver. In the testis, localized primarily in Sertoli cells. Expressed in brain with high levels in oligodendrocytes and olfactory ensheathing glia. Moderate levels of expression in astrocytes.
  • 數(shù)據(jù)庫鏈接：


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