Recombinant Mouse Growth/differentiation factor 5 (Gdf5)

中文名稱：

小鼠Gdf5重組蛋白
貨號：

CSB-EP009349MO
規格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

Gdf5
Uniprot No.：

P43027
別名：

Gdf5; Bmp14; Bp; Gdf-5Growth/differentiation factor 5; GDF-5; Bone morphogenetic protein 14; BMP-14
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length of Mature Protein
來源：

E.coli
分子量：

17.6kDa
表達區域：

376-495aa
氨基酸序列

APLANRQGKRPSKNLKARCSRKALHVNFKDMGWDDWIIAPLEYEAFHCEGLCEFPLRSHLEPTNHAVIQTLMNSMDPESTPPTCCVPTRLSPISILFIDSANNVVYKQYEDMVVESCGCR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Growth factor involved in bone and cartilage formation. During cartilage development regulates differentiation of chondrogenic tissue through two pathways. Firstly, positively regulates differentiation of chondrogenic tissue through its binding of high affinity with BMPR1B and of less affinity with BMPR1A, leading to induction of SMAD1-SMAD5-SMAD8 complex phosphorylation and then SMAD protein signaling transduction. Secondly, negatively regulates chondrogenic differentiation through its interaction with NOG. Required to prevent excessive muscle loss upon denervation. This function requires SMAD4 and is mediated by phosphorylated SMAD1/5/8. Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes.
基因功能參考文獻：
1. Microarray analysis of satellite cells showed that expression of growth differentiation factor 5 (Gdf5) mRNA was markedly increased in Dnmt3a-KO mice. The DNA methylation level of the Gdf5 promoter was markedly decreased in Dnmt3a-KO satellite cells. PMID: 29146735
2. spatiotemporal dynamics of Gdf5 expression may instruct lineage divergence. PMID: 27292641
3. he large array of modular enhancers for Gdf5 provide a new foundation for studying the spatial specificity of joint patterning in vertebrates, as well as new candidates for regulatory regions that may also influence osteoarthritis risk in human population PMID: 27902701
4. Growth differentiation factor 5 is a novel target-derived factor that promotes sympathetic axon growth and branching and makes a distinctive regional contribution to the establishment of sympathetic innervation. PMID: 26878848
5. Dach2 and Hdac9 mediate the effects of muscle activity on muscle reinnervation; Myog and Gdf5 appear to stimulate muscle reinnervation through parallel pathways PMID: 26483211
6. Clonal expansion of Gdf5 progenitors contributes to linear growth of the enthesis. PMID: 26141957
7. GDF5 might play a critical role in 3T3-L1 preadipocyte differentiation PMID: 25078108
8. These results suggest that PI3K/Akt signals play a role in the GDF5-mediated brown adipogenesis through a mechanism related to activation of the Smad pathway. PMID: 24944017
9. These results suggest that brown adipogenesis and energy homeostasis are both positively regulated by the GDF5/BMPR/Smad/PGC-1alpha signaling pathway in adipose tissues. PMID: 24062245
10. apical and basal dendritic arbours of pyramidal cells throughout the hippocampus were stunted in both homozygous and heterozygous Gdf5 null mutants, indicating that dendrite size and complexity are sensitive to the level of endogenous GDF5 synthesis. PMID: 24173804
11. This work implicates SOX11 as a potential regulator of GDF5 expression in joint maintenance and suggests a possible role in the pathogenesis of osteoarthritis PMID: 23356643
12. A novel molecular mechanism of a GDF5 mutation affecting chondrogenesis and osteogenesis, is reported. PMID: 21976273
13. Data show that revealed notochord cells in Gdf-5-null mice correctly form nuclei pulposi. PMID: 21278629
14. Although GDF-5 deficiency did not compromise long-term fracture healing, a delay in cartilage formation and remodeling supports roles for GDF-5 in the early phase of bone repair. PMID: 21590487
15. data suggest that decreased GDF5 levels in mice can contribute to osteoarthritis development by different mechanisms including altered loading and subchondral bone changes. PMID: 20805298
16. potential target gene of HOXA13 PMID: 20034107
17. These observations indicate that GDF-5 regulates differentiation of both dental papilla and follicle during odontogenesis, co-operatively with other growth factors such as BMP-2. PMID: 19909214
18. GDF5 deficiency caused a 17% increase in medium diameter (100-225 nm) collagen fibrils in tail tendon, at the expense of larger fibrils. Thus, GDF5 may play a role in tendon homeostasis in mice. PMID: 11913489
19. GDF-5-deficient femora were weaker (-31%) and more compliant (-57%) than controls when tested to failure in torsion. PMID: 11996912
20. GDF-5 induced cell cycle arrest in the G1 phase before the appearance of apoptosis in mouse B cell hybridoma HS-72 cells PMID: 12464389
21. role of single and double mutations in the mouse Gdf6 and Gdf5 genes in multiple joint and skeletal patterning defects PMID: 12606286
22. GDF5 regulates expression of connexin 43 promoter in osteoblasts ans embryos PMID: 12881039
23. GDF-5 may play an important role in modulating tendon repair. Data are consistent with previously posited roles for GDF-5 in cell recruitment, migration/adhesion, differentiation, proliferation, and angiogenesis. PMID: 12919870
24. concordance between the mRNA expression profiles of GDF5 and the gap junction gene, Cx43, in the mouse embryonic limb, spine, and heart, consistent with coordinated functions for these gene products during developmental organogenesis PMID: 14613311
25. GDF-5 synergistically enhances de novo bone formation capability of bone marrow mesenchymal cells in hyaluronan composites in rats. PMID: 14661262
26. GDF5 has a role in growth of developing joints, including early joint interzones, adult articular cartilage, and the joint capsule PMID: 15492776
27. Excessive apoptosis in the absence of GDF5 results in developmental failure of the phalanges. PMID: 15542031
28. Deficiency in knockout mice affects biomechanical behavior and ultrastructure of mouse skin. PMID: 16112556
29. Results describe 2 mutations in growth and differentiation factor 5 (GDF5) that alter receptor-binding affinities. PMID: 16127465
30. Results suggest that CDMP1/GDF5 requires cleavage by two distinct proteolytic enzymes. PMID: 16829522
31. Study further highlights a critical role of GDF5 in joint formation and the development of osteoarthritis (OA), and this should serve as a good model for OA. PMID: 17656374
32. association of Gdf5-mediated signaling pathways with Trps1 and the phenotypic changes of ATDC5 cells due to over-expression or suppression of Trps1 PMID: 18363966
33. Absence of GDF5 does not interfere with lipopolysaccharides toll-like receptor signaling in a mouse model of arthritis. PMID: 19604444
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相關疾病：

Defects in Gdf5 are the cause of brachypodism (bp) which alters the length and numbers of bones in the limbs but spares the axial skeleton.
亞細胞定位：

Secreted. Cell membrane.
蛋白家族：

TGF-beta family
數據庫鏈接：

KEGG: mmu:14563

STRING: 10090.ENSMUSP00000048079

UniGene: Mm.4744