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  4. Recombinant Mouse Glycerol-3-phosphate acyltransferase 2, mitochondrial (Gpat2), partial

Recombinant Mouse Glycerol-3-phosphate acyltransferase 2, mitochondrial (Gpat2), partial

  • 中文名稱：
    小鼠Gpat2重組蛋白
  • 貨號：
    CSB-YP623957MO
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Gpat2重組蛋白
  • 貨號：
    CSB-EP623957MO
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Gpat2重組蛋白
  • 貨號：
    CSB-EP623957MO-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Gpat2重組蛋白
  • 貨號：
    CSB-BP623957MO
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Gpat2重組蛋白
  • 貨號：
    CSB-MP623957MO
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Gpat2
  • Uniprot No.：
  • 別名：
    Gpat2; Glycerol-3-phosphate acyltransferase 2; mitochondrial; GPAT-2; EC 2.3.1.15; xGPAT1
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Transfers an acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate producing a lysophosphatidic acid (LPA), an essential step for the triacylglycerol (TAG) and glycerophospholipids. In vitro also transfers an acyl-group from acyl-ACP to the LPA producing a phosphatidic acid (PA). Prefers arachidonoyl-CoA as the acyl donor. Required for primary processing step during piRNA biosynthesis. Molecular mechanisms by which it promotes piRNA biosynthesis are unclear and do not involve its acyltransferase activity.
  • 基因功能參考文獻(xiàn)：
    1. Data suggest that Gpat2 is necessary for normal spermatogenesis; absence of Gpat2 up-regulates expression of apoptosis regulatory proteins, triggers apoptosis, and thereby decreases the number of dividing germ cells; Gpat2 knockdown (using RNA silencing) prevents germ cells from proceeding through pachytene stage. PMID: 28729426
    2. Thus, Gpat2 is expressed at a specific stage of spermatogenesis, consistent with piRNA synthesis and meiosis I prophase PMID: 26268560
    3. GPAT2 is essential for the biogenesis of piRNA. PMID: 23611983
    4. GPAT2 expression is linked to arachidonoyl-CoA incorporation into TAG in spermatogenic germ cells PMID: 22905194
    5. These results suggest that the GPAT2 protein has two transmembrane segments and that the N-terminal and C-terminal regions of this protein face the cytoplasm. PMID: 22285183
    6. A novel murine glycerol-3-phosphate acyltransferase-like protein 1 (named xGPAT1) has been cloned. PMID: 17013544

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  • 亞細(xì)胞定位：
    Mitochondrion outer membrane; Multi-pass membrane protein.
  • 蛋白家族：
    GPAT/DAPAT family
  • 組織特異性：
    Highly expressed in the testis. Expressed at lower levels in the heart, liver, kidney, spleen and adipose cells. Only detected in primary spermatocytes.
  • 數(shù)據(jù)庫鏈接：


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