Recombinant Mouse Gasdermin-A3 (Gsdma3), partial

^{In Stock}

中文名稱：

小鼠Gsdma3重組蛋白
貨號：

CSB-EP730416MO1
規格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

Gsdma3
Uniprot No.：

Q5Y4Y6
別名：

(Gasdermin-3)(GSDMA3-NT)(GSDMA3-CT)
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
來源：

E.coli
分子量：

26.6 kDa
表達區域：

263-464aa
氨基酸序列

MHEDFKTLKEEVQRETQEVEKLSPVGRSSLLTSLSHLLGKKKELQDLEQKLEGALDKGQKVTLEALPKDVLLSKDAMDAILYFLGALTELTEEQLKILVKSLEKKILPVQLKLVESTLEQNFLQDKEGVFPLQPDLLSSLGEEELTLTEALVGLSGLEVQRSGPQYAWDPDTRHNLCALYAGLSLLHLLSRKSNALTYCALS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Precursor of a pore-forming protein involved in the transition from catagen to telogen at the end of hair follicle morphogenesis. This form constitutes the precursor of the pore: upon cleavage, the released N-terminal moiety (Gasdermin-A3, N-terminal) binds to membranes and forms pores, triggering cell death.; Pore-forming protein that causes membrane permeabilization and pyroptosis. Released upon cleavage in vitro of genetically engineered Gsdma3, and binds to membrane inner leaflet lipids. Homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, triggering pyroptosis. Binds to membrane inner leaflet lipids, including bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate, as well as phosphatidylinositol (3,4,5)-bisphosphate, and more weakly to monophosphorylated phosphatidylinositols. Also binds to bacterial and mitochondrial lipids, including cardiolipin, and exhibits bactericidal activity. The functional mechanisms and physiological proteases that cleave and activate this pore-forming protein are unknown (Probable). Plays a role in the transition from catagen to telogen at the end of hair follicle morphogenesis, possibly by regulating hair follicle stem cell niche maintenance. Also required for mammary gland development.
基因功能參考文獻：
1. cryo-electron microscopy structures of the 27-fold and 28-fold single-ring pores formed by the N-terminal fragment of mouse GSDMA3 (GSDMA3-NT) at 3.8 and 4.2 A resolutions, and of a double-ring pore at 4.6 A resolution PMID: 29695864
2. In this study, we describe a novel mutation (C+/H-: cornea plus, hair minus) in the far C-terminal region of Gasdermin A3 that causes both skin and corneal phenotypes. PMID: 29372254
3. The function of Gsdma3 in regulating the development of mammary glands, and it demonstratse that the Gsdma3 gene may act as a suppressor of the immune reaction. PMID: 28168650
4. induced expression caused epidermal hyperplasia, skin inflammation and hair cycle defect, suggesting gain-of-function mutations PMID: 26173759
5. crystal structure of GSDMA3 showed an autoinhibited two-domain architecture that is conserved in the gasdermin family; the gasdermin-N domain is required for pyroptosis PMID: 27281216
6. Our results indicate that the genetic mutations in the C-terminal domain of Gsdma3 are gain-of-function mutations which unmask the N-terminal functional domain of Gsdma3 PMID: 26100518
7. GSDMA3 mutation play a specific role in hair growth in alopecia. PMID: 25860385
8. Loss of conserved Gsdma3 self-regulation causes autophagy and cell death. PMID: 25825937
9. The Gsdma3 may play a role in the proliferation and differentiation of epidermal cells and hair follicles through negatively regulating beta-catenin expression. PMID: 22694914
10. in mouse skin keratinocytes, Gsdma3 expression could be regulated by TNF-alpha. PMID: 22585037
11. Gsdma3(I359N) is a novel ENU-induced mutant mouse line for studying the function of Gasdermin A3 in the hair follicle and epidermis PMID: 22682752
12. The results demonstrate that Gsdma3 causes immune-mediated destruction of bulge stem cells plays a crucial role in the pathogenesis of alopecia in AE mice, and this strain might be implicated in other PCAs including lichen planopilaris. PMID: 22155111
13. Gsdma3 gene mutation interfered with catagen formation during mouse hair follicle cycle and, by upregulation of Caspase-3 expression and promotion of apoptosis, Gsdma3 gene could play an essential role in normal catagen induction. PMID: 21805153
14. These results suggest that Gsdma3 is necessary in hair shaft differentiation, and involved in Msx2 regulatory pathway. PMID: 20977888
15. Gsdm3 reflects a mutation hotspot and that Gsdm3 mutations cause alopecia in Rco2, Re(den), and Bsk mice. PMID: 15475261
16. role for gasdermins in differentiation of the epidermis and its appendages. PMID: 15737203
17. These results suggest that Gsdma3 is involved in the proliferation and differentiation of epidermal stem cells. PMID: 17572385
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相關疾病：

Defects in Gsdma3 are the cause of a number of alopecia phenotypes, bareskin (Bsk), defolliculated (Dfl), finnegan (Fgn) reduced coat 2 (Rco2), Rex-denuded (Re-den) and recombination induced mutation 3 (Rim3). These are dominant conditions characterized by loss of hair.
亞細胞定位：

[Gasdermin-A3]: Cytoplasm, cytosol.; [Gasdermin-A3, N-terminal]: Cell membrane; Multi-pass membrane protein. Mitochondrion membrane; Multi-pass membrane protein.
蛋白家族：

Gasdermin family
組織特異性：

Highest levels in skin with weak expression in placenta and testis. Not detected in the gastrointestinal tract. In skin, expressed in postnatal hair follicles and epidermis as well as sebaceous gland basal cells.
數據庫鏈接：

KEGG: mmu:450219

UniGene: Mm.371065