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  4. Recombinant Mouse E3 ubiquitin-protein ligase RFWD3 (Rfwd3)

Recombinant Mouse E3 ubiquitin-protein ligase RFWD3 (Rfwd3)

  • 中文名稱：
    小鼠Rfwd3重組蛋白
  • 貨號：
    CSB-YP811879MO
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Rfwd3重組蛋白
  • 貨號：
    CSB-EP811879MO
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Rfwd3重組蛋白
  • 貨號：
    CSB-EP811879MO-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Rfwd3重組蛋白
  • 貨號：
    CSB-BP811879MO
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Rfwd3重組蛋白
  • 貨號：
    CSB-MP811879MO
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Rfwd3
  • Uniprot No.：
  • 別名：
    Rfwd3; Rnf201; E3 ubiquitin-protein ligase RFWD3; EC 2.3.2.27; RING finger and WD repeat domain-containing protein 3; RING finger protein 201
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-774
  • 氨基酸序列
    MAEVAVGQAS DLVPSEMDHE VIYSHLQGPL EGTIEPATPT EVVSNGAPLQ PAPAELANSQ GGAHVQPAPA EVVSSQDGLP TLQPLPPASI DLTEEVQPSE ENMEVVNPGT SEEPSQGSGA NPTAGAARSV SMNNFISGLQ RLHNMLELLR PPPADHSVGP IRTRRRMAPI LRARAGESQR QDNGRYVPHT PLYAYFQVSR SQPYPLPAAH DSETRNPPSG TDSDSDGSAE DEEVVVQAEE PEANIPEQGV IATDQEATSV TGDDAVPKES PQKPNMLSAM EDEEGETCTI CLEQWTNAGD HRISALRCGH LFGFRCISKW LKGQTRKCPQ CNKKAKHSDI VVIYARSLRA LDTSEQERMK SDLLNEQMLR KQAELESAQC RLQLQVLIDK CTKLNSRVQD LEKFVVRHQN SAAQQCSRSK ALCGKCLSSS QSPGKYSSEK TFTISQTGKC RILAYCDGLS CLVASQPSPQ ASFLPGFGVK MLSTANMKSS QYIPMHNKQI RGLSFSSQSK GLLLSASLDC TIKLTSLETN TVVQTYNTGR PVWSCCWCLD ENNYVYAGLA SGSILIYDLR NTNTYIQELV PQKARCPLVS LSYLPKAAVA AFPYGGVLAG TLENASFWEL KSDFSHKPHV LSLEPGGFVD FQTESSTRHC LVTYRPDKSH NTVRTVLLEM SYKLDDAGEP VCSCLPVQTF LGGPTCKLLT KSAIFQSPEN NGNVLVCTGD EASQSTLLWN AGSGLLLQNL KAGEPVLDIC PFETNQNSYL ATLTEKTVHM YKWE
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage. Plays a key role in RPA-mediated DNA damage signaling and repair. Acts by mediating ubiquitination of the RPA complex (RPA1, RPA2 and RPA3 subunits) and RAD51 at stalled replication forks, leading to remove them from DNA damage sites and promote homologous recombination. Also mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53.
  • 亞細胞定位：
    Nucleus. Nucleus, PML body. Cytoplasm.
  • 數據庫鏈接：


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