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  4. Recombinant Mouse Dimethyladenosine transferase 2, mitochondrial (Tfb2m)

Recombinant Mouse Dimethyladenosine transferase 2, mitochondrial (Tfb2m)

  • 中文名稱：
    小鼠Tfb2m重組蛋白
  • 貨號：
    CSB-YP666227MO
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Tfb2m重組蛋白
  • 貨號：
    CSB-EP666227MO
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Tfb2m重組蛋白
  • 貨號：
    CSB-EP666227MO-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Tfb2m重組蛋白
  • 貨號：
    CSB-BP666227MO
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Tfb2m重組蛋白
  • 貨號：
    CSB-MP666227MO
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Tfb2m
  • Uniprot No.：
  • 別名：
    Tfb2m; Dimethyladenosine transferase 2; mitochondrial; EC 2.1.1.-; Mitochondrial 12S rRNA dimethylase 2; Mitochondrial transcription factor B2; mTFB2M; mtTFB2; S-adenosylmethionine-6-N'; N'-adenosyl(rRNA) dimethyltransferase 2
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    44-396
  • 氨基酸序列
    SDFNIEP LPDSDLEESS PWTSRNRSEP TRHIACKKAA RNLVRDLLEH QNPSRQIILE CNPGPGILTG ALLKAGARVV AFESEKTFIP HLEPLQRNMD GELQVVHCDF FKMDPRYQEV VRPDVSSQAI FQNLGIKAVP WSAGVPIKVF GILPYKHERR ILWKILFDLY SCESIYRYGR VELNMFVSEK EFRKLIATPK RPDLYQVMAV LWQVACDVKF LHMEPWSSFS VHTENGHLEK SKHGESVNLL KQNLYLVRMT PRRTLFTENL SPLNYDIFFH LVKHCFGKRN APIIRHLRSL STVDPINILR QIRKNPGDTA ARMYPHDFKK LFETIEQSED SVFKWIYDYC PEDMEF
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    S-adenosyl-L-methionine-dependent rRNA methyltransferase which may methylate two specific adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 12S mitochondrial rRNA. Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA. In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. Stimulates transcription independently of the methyltransferase activity.
  • 基因功能參考文獻：
    1. Loss of Tfb2m in pancreatic beta-cells results in progressive mitochondrial dysfunction and impaired insulin secretion in response to metabolic stimuli. PMID: 28702322
    2. Metazoans have two TFBM genes, suggesting that a duplication of the gene has permitted a more flexible regulation of mtDNA. PMID: 12532263
  • 亞細胞定位：
    Mitochondrion.
  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, rRNA adenine N(6)-methyltransferase family, KsgA subfamily
  • 組織特異性：
    Ubiquitously expressed.
  • 數據庫鏈接：


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