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  2. 蛋白
  3. 重組蛋白
  4. Recombinant Mouse DNA nucleotidylexotransferase (Dntt)

Recombinant Mouse DNA nucleotidylexotransferase (Dntt)

  • 中文名稱：
    小鼠Dntt重組蛋白
  • 貨號(hào)：
    CSB-YP007088MO
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Dntt重組蛋白
  • 貨號(hào)：
    CSB-EP007088MO
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Dntt重組蛋白
  • 貨號(hào)：
    CSB-EP007088MO-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Dntt重組蛋白
  • 貨號(hào)：
    CSB-BP007088MO
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Dntt重組蛋白
  • 貨號(hào)：
    CSB-MP007088MO
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Dntt; Tdt; DNA nucleotidylexotransferase; EC 2.7.7.31; EC 3.1.11.-; Terminal addition enzyme; Terminal deoxynucleotidyltransferase; TDT; Terminal transferase
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長(zhǎng)度：
    Full length protein
  • 表達(dá)區(qū)域：
    1-530
  • 氨基酸序列
    MDPLQAVHLG PRKKRPRQLG TPVASTPYDI RFRDLVLFIL EKKMGTTRRA FLMELARRKG FRVENELSDS VTHIVAENNS GSDVLEWLQL QNIKASSELE LLDISWLIEC MGAGKPVEMM GRHQLVVNRN SSPSPVPGSQ NVPAPAVKKI SQYACQRRTT LNNYNQLFTD ALDILAENDE LRENEGSCLA FMRASSVLKS LPFPITSMKD TEGIPCLGDK VKSIIEGIIE DGESSEAKAV LNDERYKSFK LFTSVFGVGL KTAEKWFRMG FRTLSKIQSD KSLRFTQMQK AGFLYYEDLV SCVNRPEAEA VSMLVKEAVV TFLPDALVTM TGGFRRGKMT GHDVDFLITS PEATEDEEQQ LLHKVTDFWK QQGLLLYCDI LESTFEKFKQ PSRKVDALDH FQKCFLILKL DHGRVHSEKS GQQEGKGWKA IRVDLVMCPY DRRAFALLGW TGSRQFERDL RRYATHERKM MLDNHALYDR TKGKTVTISP LDGKVSKLQK ALRVFLEAES EEEIFAHLGL DYIEPWERNA
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Transferase that catalyzes the nontemplated addition of nucleoside triphosphate to coding ends during V(D)J recombination (N addition). Involved in the generation of diversity in the antigen-binding region of immunoglobulin heavy and light chains and T-cell receptors during B- and T-cell development. Does not act on double-stranded DNA with blunt ends.; 3'-to-5' DNA exonuclease. Involved in the generation of diversity in the antigen-binding region of immunoglobulin heavy and light chains and T-cell receptors during B- and T-cell development. Acts on single-stranded and double-stranded DNA with 3' or 5' extensions, but not on double-stranded DNA with blunt ends. Attenuates not only isoform TDT-S-catalyzed N addition, but also P (palindromic) addition in coding joins. Lacks terminal transferase activity.
  • 基因功能參考文獻(xiàn)：
    1. Molecular dynamics simulations and structural analysis to decipher functional impact of a twenty residue insert in the ternary complex of mus musculus TdT isoform have been reported. PMID: 27311013
    2. TdT is essential for the generation of the predominant higher-affinity DEX-responsive J558 clone PMID: 20018621
    3. Production of M603-id antibody is TdT dependent, while generation of M167-id antibody is TdT independent. PMID: 11932921
    4. onset of terminal deoxynucleotidyl transferase gene activation during T cell differentiation and thymic ontogeny PMID: 12516554
    5. The role of this locus in autoantibody production in MRL/lpr mouse strain, and subsequent development of kidney disease. PMID: 12804532
    6. tdt may be involved in learning and memory saving. PMID: 12821797
    7. There is an observed decrease in the incidence of autoimmune disease, including absence of diabetes and decreased pancreatic infiltration in NOD TdT-deficient mice, and reduced glomerulonephritis and increased life span in MRL-Fas(lpr) TdT-deficient mice. PMID: 15034081
    8. Structure-function analysis of proposed domains and motifs for known activity of TdT reveals nonessential role for tumor suppressor BRCA1 C-terminal (BRCT) domain and essential roles for second helix-hairpin-helix motif and entire C terminus of TdT. PMID: 15100289
    9. third hypervariable region assumes for each immunoglobulin chain, with DNA polymerase (pol) lambda maintaining a large heavy chain junctional heterogeneity and pol mu ensuring a restricted light chain junctional variability PMID: 16860755
    10. TdT plays a critical role in the magnitude and breadth of anti-viral T(CD8+) responses toward individual determinants PMID: 18566432
    11. The role of TdT ensures enhanced diversity and selection of private T cell receptor repertoires and promotes optimal CD8-positive T cell immunity, both within individuals and across the species as a whole. PMID: 18684946

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  • 亞細(xì)胞定位：
    [Isoform TDT-S]: Nucleus.; [Isoform TDT-L]: Nucleus. Cytoplasm.
  • 蛋白家族：
    DNA polymerase type-X family
  • 組織特異性：
    Isoform TDT-L: Expressed in the thymus, and, at lower levels, in the bone marrow. Detected in both cycling and noncycling pro-B and pre-B cells (at protein level). Isoform TDT-S: Expressed in both cycling and noncycling pro-B, but not pre-B, cells (at pro
  • 數(shù)據(jù)庫(kù)鏈接：


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