在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. 首頁
  2. 蛋白
  3. 重組蛋白
  4. Recombinant Mouse DNA-directed RNA polymerase II subunit RPB1 (Polr2a), partial

Recombinant Mouse DNA-directed RNA polymerase II subunit RPB1 (Polr2a), partial

  • 中文名稱：
    小鼠Polr2a重組蛋白
  • 貨號：
    CSB-YP018327MO
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Polr2a重組蛋白
  • 貨號：
    CSB-EP018327MO
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Polr2a重組蛋白
  • 貨號：
    CSB-EP018327MO-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Polr2a重組蛋白
  • 貨號：
    CSB-BP018327MO
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Polr2a重組蛋白
  • 貨號：
    CSB-MP018327MO
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Polr2a; Rpii215; Rpo2-1; DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; EC 2.7.7.6; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Regulation of gene expression levels depends on the balance between methylation and acetylation levels of tha CTD-lysines. Initiation or early elongation steps of transcription of growth-factors-induced immediate early genes are regulated by the acetylation status of the CTD. Methylation and dimethylation have a repressive effect on target genes expression.
  • 基因功能參考文獻：
    1. significantly higher binding of POLR2A to the Tmem132d promoter region of high anxiety behavior mice than in low anxiety behavior mice PMID: 29317594
    2. Skill learning modulates RNA Pol II poising at immediate early genes in the adult striatum. These experiments demonstrate a novel phenomenon of learning induced transcriptional modulation in adult brain. PMID: 28451632
    3. An H3K9/S10 methyl-phospho switch modulates Polycomb and Pol II binding at repressed genes during differentiation. PMID: 24430871
    4. Cdc14b regulates mammalian RNA polymerase II and represses cell cycle transcription PMID: 22355704
    5. These data indicate that, through cooperation with PolII and KDM6A, Spt6 orchestrates removal of H3K27me3, thus controlling developmental gene expression and cell differentiation. PMID: 23503590
    6. Data show that that B2 RNA, when present with Pol II in promoter-bound complexes, specifically represses CTD phosphorylation by TFIIH. PMID: 21326911
    7. investigation of contribution of largest subunit of phosphorylated RNAP II to onset of minor gene activation in zygote; nuclear localization of RNAP II in 1-cell zygote; regulation of RNAP II expression by deposition of acetylated histone on promoter PMID: 20710121
    8. involvement of RPB1, the largest subunit of RNA polymerase II (RNAP II), in the regulation of transcriptional silencing mechanism PMID: 20562521
    9. These results indicate that Wwp2 plays an important role in regulating expression of Rpb1 in normal physiological conditions.[Rpb1] PMID: 17526739
    10. Results suggest that RNA polymerase II is selectively required for positive transcriptional regulation of a subset of genes in embryonic stem cells. PMID: 17892859
    11. The authors that both the ubiquitylation and proteasomal degradation of the largest subunit of pol II (Rpb1) following UV-irradiation are significantly suppressed in Elongin A-deficient cells. PMID: 19037258

    顯示更多

    收起更多

  • 亞細胞定位：
    Nucleus. Cytoplasm. Chromosome.
  • 蛋白家族：
    RNA polymerase beta' chain family
  • 數據庫鏈接：


主站蜘蛛池模板： 青青草无码精品伊人久久| 亚洲精品国产成人一区二区| 无码国产玉足脚交极品播放| 亚洲精品国偷自产在线99人热 | 亚洲一区 日韩精品 中文字幕| 任你干视频精品播放| 久青青在线观看视频国产| 精品亚洲成a人无码成a在线观看 | 少妇被又大又粗又爽毛片久久黑人| 欧美刺激性大交| 久久精品国产99久久丝袜蜜桃| 色偷偷www.8888在线观看| 免费国产裸体美女视频全黄| 亚洲熟妇无码爱v在线观看| 亚洲码国产精品高潮在线| 久久成人影院精品99| 日本日本乱码伦专区| 激情综合一区二区三区| 国产欧美熟妇另类久久久| 无遮挡午夜男女xx00动态| 午夜免费啪视频观看视频| 国产熟妇按摩3p高潮大叫| 精品乱码久久久久久中文字幕| 青草av久久一区二区三区| 吃奶呻吟张开双腿做受在线播放| 国产精品亚洲а∨天堂2021| 天天看片视频免费观看| 国内精品久久久久久影院8f| 色偷偷色噜噜狠狠网站久久| 精品国产不卡一区二区三区| 国产成人精品午夜视频| 综合亚洲综合图区网友自拍| 国偷自产av一区二区三区| 亚洲性人人天天夜夜摸| 日日碰狠狠添天天爽五月婷| 中国孕妇变态孕交xxxx| 亚洲人成在线观看网站不卡| 欧美日本特级婬片视频| 国产奶头好大揉着好爽视频| 麻豆久久久9性大片| 337p日本大胆欧美裸体艺术|