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  4. Recombinant Mouse Cytoplasmic protein NCK2 (Nck2)

Recombinant Mouse Cytoplasmic protein NCK2 (Nck2)

  • 中文名稱：
    小鼠Nck2重組蛋白
  • 貨號：
    CSB-YP015531MO
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Nck2重組蛋白
  • 貨號：
    CSB-EP015531MO
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Nck2重組蛋白
  • 貨號：
    CSB-EP015531MO-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Nck2重組蛋白
  • 貨號：
    CSB-BP015531MO
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Nck2重組蛋白
  • 貨號：
    CSB-MP015531MO
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Nck2; Grb4; Cytoplasmic protein NCK2; Growth factor receptor-bound protein 4; NCK adaptor protein 2; Nck-2; SH2/SH3 adaptor protein NCK-beta
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-380
  • 氨基酸序列
    MTEEVIVIAK WDYTAQQDQE LDIRKNERLW LLDDSKTWWR VRNAANRTGY VPSNYVERKN SLKKGSLVKN LKDTLGLGKT RRKPSARDAS PTPSTDAEYP ANGSGADRIY DLNIPAFVKF AYVAEREDEL SLVKGSRVTV MEKCSDGWWR GSFNGQIGWF PSNYVLEEAD EAAAEAPSFL SLRRGTALSN GQGARVLHVV QTLYPFSSVT EEELSFEKGE TMEVIEKPEN DPEWWKCKNA RGQVGLVPKN YVVVLSDGPA LHPAHTPQIS YTGPSASGRF AGREWYYGNV TRHQAECALN ERGVEGDFLI RDSESSPSDF SVSLKASGRN KHFKVQLVDS VYCIGQRRFH SMDELVEHYK KAPIFTSEHG EKLYLVRALQ
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.
  • 基因功能參考文獻：
    1. Authors propose that Nck2 should be further investigated as a regulator of the reliance of white adipose tissue on hyperplasia versus hypertrophy during adipose tissue expansion, and hence, as a potential novel molecular target in obesity. PMID: 28425845
    2. Findings reveal that Nck2 is a novel regulator of adiposity and suggest that Nck2 is important in limiting WAT expansion and dysfunction in mice and humans. PMID: 27325288
    3. Inactivation of NCK2 (and NCK1) inhibits endothelial cell polarity. PMID: 26659946
    4. Data show that only Nck2 is required for the Slit1-induced changes in cortical neuron morphology in vitro. PMID: 21600986
    5. These results revealed an essential role for HSF4-mediated SKAP2 expression in the regulation of actin reorganization during lens differentiation. PMID: 20219016
    6. The Cas utilizes Nck2 to activate Cdc42 and induce cell polarization in response to wound healing. PMID: 20637038
    7. Nck beta interacts with tyrosine-phosphorylated disabled 1 and redistributes in Reelin-stimulated neurons PMID: 14517291
    8. Grb4 is a key mediator for transducing ephrin B3 reverse signals involved in axon pruning in cultured hippocampal granule cell neurons. PMID: 19182796
    9. Transduces B-ephrin reverse signals. PMID: 11557983

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  • 亞細胞定位：
    Cytoplasm. Endoplasmic reticulum.
  • 數據庫鏈接：


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