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  4. Recombinant Mouse Coiled-coil domain-containing protein 111 (Ccdc111)

Recombinant Mouse Coiled-coil domain-containing protein 111 (Ccdc111)

  • 中文名稱：
    小鼠Primpol重組蛋白
  • 貨號：
    CSB-YP764710MO
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Primpol重組蛋白
  • 貨號：
    CSB-EP764710MO
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Primpol重組蛋白
  • 貨號：
    CSB-EP764710MO-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Primpol重組蛋白
  • 貨號：
    CSB-BP764710MO
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Primpol重組蛋白
  • 貨號：
    CSB-MP764710MO
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Primpol
  • Uniprot No.：
  • 別名：
    Primpol; DNA-directed primase/polymerase protein; EC 2.7.7.-
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-537
  • 氨基酸序列
    MLRKWEARVK QIEERASHYE RKPLSSVYRP RLAKPEEPSS IWKLFHRQNQ AFNFVKSCKE SVHVFALECK RGNGQRIYLV TSYAQLWFYY KTRKTLLHCY EVIPENAVCK LYFDLEFNKL ANPGADGKMM VALLIQHVCK ALEEFYNVQC SAEDVFNLDS STEEKFSRHL IFQLHNVAFK DNRHAGNFVR KILQPALHLI AEDDEAKVPE AVGQDASGFS VTPLKQEISE AREKVGLPKQ CDPDLSFLVV KNHMGEKCLF VDLGVYTKNR NFRLYQSSKI GKCVSLEVAE DNRFIPKQSK DISEENQYFL SSLVSNVRFS DTLRVLTCHP SQTKRKRAEC FNSTGTSVES IEGFQGSPYP EVDQFVLSLV NKHDIKGGIR RWNYFFPEEL LVYDICKYRW CENIGRAHKS NNIMILVDLK NEVWYQKCHD PVCKAQNFKS TCSPLPTEVS LLFLLKDEDF TSGETDDTST SLTKDSQTPP SCNLSAGGLS AAAWDDEDDA LFLEATEDAE FADAADKSLG SMDDIPDELI IEALQNS
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    DNA primase and DNA polymerase required to tolerate replication-stalling lesions by bypassing them. Required to facilitate mitochondrial and nuclear replication fork progression by initiating de novo DNA synthesis using dNTPs and acting as an error-prone DNA polymerase able to bypass certain DNA lesions. Shows a high capacity to tolerate DNA damage lesions such as 8oxoG and abasic sites in DNA. Provides different translesion synthesis alternatives when DNA replication is stalled: able to synthesize DNA primers downstream of lesions, such as ultraviolet (UV) lesions, R-loops and G-quadruplexes, to allow DNA replication to continue. Can also realign primers ahead of 'unreadable lesions' such as abasic sites and 6-4 photoproduct (6-4 pyrimidine-pyrimidinone), thereby skipping the lesion. Also able to incorporate nucleotides opposite DNA lesions such as 8oxoG, like a regular translesion synthesis DNA polymerase. Also required for reinitiating stalled forks after UV damage during nuclear DNA replication. Required for mitochondrial DNA (mtDNA) synthesis and replication, by reinitiating synthesis after UV damage or in the presence of chain-terminating nucleotides. Prevents APOBEC family-mediated DNA mutagenesis by repriming downstream of abasic site to prohibit error-prone translesion synthesis. Has non-overlapping function with POLH. In addition to its role in DNA damage response, also required to maintain efficient nuclear and mitochondrial DNA replication in unperturbed cells.
  • 基因功能參考文獻：
    1. PrimPol can reinitiate stalled mtDNA replication and can prime mtDNA replication from nonconventional origins. PMID: 29073063
  • 亞細胞定位：
    Nucleus. Mitochondrion matrix. Chromosome.
  • 蛋白家族：
    Eukaryotic-type primase small subunit family
  • 數據庫鏈接：


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