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  4. Recombinant Mouse Centrin-1 (Cetn1)

Recombinant Mouse Centrin-1 (Cetn1)

  • 中文名稱：
    Recombinant Mouse Centrin-1(Cetn1)
  • 貨號：
    CSB-YP005264MO
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Mouse Centrin-1(Cetn1)
  • 貨號：
    CSB-EP005264MO
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Mouse Centrin-1(Cetn1)
  • 貨號：
    CSB-EP005264MO-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Mouse Centrin-1(Cetn1)
  • 貨號：
    CSB-BP005264MO
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Mouse Centrin-1(Cetn1)
  • 貨號：
    CSB-MP005264MO
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Cetn1
  • Uniprot No.：
  • 別名：
    Cetn1; Calt; Centrin-1; Caltractin
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Full length protein
  • 表達區域：
    1-172
  • 氨基酸序列
    MASTFRKSNV ASTSYKRKVG PKPELTEDQK QEVREAFDLF DSDGSGTIDV KELKVAMRAL GFEPRKEEMK KMISEVDKEA TGKISFNDFL AVMTQKMAEK DTKEEILKAF RLFDDDETGK ISFKNLKRVA NELGESLTDE ELQEMIDEAD RDGDGEVNEE EFLKIMKKTN LY
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Plays a fundamental role in microtubule-organizing center structure and function. Plays a role in sperm cilia formation.
  • 基因功能參考文獻：
    1. The crystal structure exhibits three structural features, i.e. four Ca2+ equally occupied at each EF-hand motif, structural changes accompanying helix motion at the N- and C-lobes, and adoption of N-C type dimerization when Ca2+ binds to EF-hand I and II in the N-lobe. PMID: 29037133
    2. Experiments utilizing mouse embryo fibroblasts (MEFs) demonstrated the feasibility of real-time imaging, and showed that EGFP-Centrin1 expression mirrored the endogenous centrosome cycle, undergoing precisely one round of duplication through the cell cycle. Moreover, experiments using embryo and adult mouse tissues demonstrated that EGFP-Centrin1 specifically mirrors the localization of endogenous centrosomes. PMID: 26990996
    3. role in late steps of spermiogenesis and spermatid maturation PMID: 23641067
    4. Immunostaining of seminiferous tubules revealed that cells in layer containing spermatogonia showed colocalization of SUMO-2/3 with centrin-1 in cytoplasmic spots. Binding of centrin-1 and SUMO-2/3 appears to be calcium dependent. PMID: 20941751
    5. Calcium-dependent assembly of centrin-G-protein complex in photoreceptor cells PMID: 11884606
    6. Cen1 and Cen2 contribute to the centrin-transducin complex and potentially participate in the regulation of transducin translocation through the photoreceptor cilium PMID: 15347651

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  • 亞細胞定位：
    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cell projection, cilium.
  • 蛋白家族：
    Centrin family
  • 組織特異性：
    Expressed in testis. Localizes to the caudal portion of spermatozoa in seminiferous tubule and epididymis. Expressed in retina photoreceptor cells (at protein level).
  • 數據庫鏈接：


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